CCL25/TECK Antibody (52529) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 52529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CCL25/TECK Antibody (52529) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human CCL25/TECK
  Gln24-Leu150
  Accession # AAB69981
• Specificity: Detects human CCL25/TECK in direct ELISAs and Western blots. Shows less than 1% cross-reactivity with recombinant mouse CCL25 and recombinant rat CINC-1 and no cross-reactivity with recombinant human (rh)CCL28, recombinant mouse (rm)CCL28, rhMPIF-1,
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody (52529) [Alexa Fluor® 488]

• C-C motif chemokine 25
• CCL25
• chemokine (C-C motif) ligand 25
• Chemokine TECK
• Ck beta-15
• Ckb15
• SCYA25TECKvar
• small inducible cytokine subfamily A (Cys-Cys), member 25
• Small-inducible cytokine A25
• TECK
• TECKMGC150327
• thymus expressed chemokine
• Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.