CCL25/TECK Antibody (52513)

Recombinant Human CCL25/TECK (Catalog # 334‑TK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL25/TECK (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL25/TECK Monoclonal Antibody (Catalog # MAB334). The ND50 is typically 3‑12 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 52513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL25/TECK Antibody (52513) Summary

• Immunogen: E. coli-derived recombinant human CCL25/TECK
  Gln24-Leu150
  Accession # AAB69981
• Specificity: Detects human CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12, 17, 19, 21, 22, or recombinant rat CCL20. is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL25
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 3-12 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody (52513)

• C-C motif chemokine 25
• CCL25
• chemokine (C-C motif) ligand 25
• Chemokine TECK
• Ck beta-15
• Ckb15
• SCYA25TECKvar
• small inducible cytokine subfamily A (Cys-Cys), member 25
• Small-inducible cytokine A25
• TECK
• TECKMGC150327
• thymus expressed chemokine
• Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor

1. Vicari, A.P. et al. (1997) Immunity 7:291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL25/TECK Antibody (MAB334)(1)

Publication using MAB334

• Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003-12-04;103(7):2806-8. 2003-12-04 [PMID: 14656893] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CCL25
• Uniprot:
  • AAB69981()