CCL24/Eotaxin-2/MPIF-2 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL24/Eotaxin-2/MPIF-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL24/Eotaxin-2/MPIF-2 (R&D Systems, Catalog # 343-E2)
  Val27-Cys119
  Accession # AAB51135
• Specificity: Detects human CCL24/Eotaxin-2/MPIF-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha , and rhMCP-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL24
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CCL24/Eotaxin-2/MPIF-2 Antibody (Catalog # MAB343)
  ELISA Detection: Human CCL24/Eotaxin-2/MPIF-2 Biotinylated Antibody (Catalog # BAF343)
  Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 (Catalog # 343-E2)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody [Biotin]

• CCL24
• chemokine (C-C motif) ligand 24
• Ckb-6
• Eosinophil chemotactic protein 2
• Eotaxin-2
• member 24
• MPIF2
• MPIF-2
• MPIF2Myeloid progenitor inhibitory factor 2
• MPIF-2Small-inducible cytokine A24
• SCYA24CK-beta-6

Background

Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin-2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy-terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42%, and 39% amino acid identity to MCP-3, MIP-1 alpha , and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.

Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR3, at least on eosinophils.

1. Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
2. Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL24/Eotaxin-2/MPIF-2 Antibody (BAF343)(1)

Publication using BAF343

• Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE Eotaxin-2 and colorectal cancer: a potential target for immune therapy. Clin. Cancer Res., 2007-10-01;13(19):5719-28. 2007-10-01 [PMID: 17908961] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL24
• Uniprot:
  • AAB51135()