CCL24/Eotaxin-2/MPIF-2 Antibody [Unconjugated]

CCL24/Eotaxin-2/MPIF-2 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human CCL24/Eotaxin-2/ MPIF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human CCL24/Eotaxin‑2 (Catalog # 343‑E2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/Eotaxin‑2 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL24/Eotaxin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343). The ND50 is typically 0.5-2.5 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL24/Eotaxin-2/MPIF-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2
  Val27-Cys119
  Accession # AAB51135
• Specificity: Detects human CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse CCL24/Eotaxin-2/MPIF-2 is observed and less than 5% cross‑reactivity with recombinant human (rh) MPIF-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL24
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody [Unconjugated]

• CCL24
• chemokine (C-C motif) ligand 24
• Ckb-6
• Eosinophil chemotactic protein 2
• Eotaxin-2
• member 24
• MPIF2
• MPIF-2
• MPIF2Myeloid progenitor inhibitory factor 2
• MPIF-2Small-inducible cytokine A24
• SCYA24CK-beta-6

Background

Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF‑2/Eotaxin‑2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy‑terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha , and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.

Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.

1. Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
2. Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL24/Eotaxin-2/MPIF-2 Antibody (AF343)(2)

Publications using AF343

• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human)
• Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities. J. Immunol., 2006-03-15;176(6):3788-95. 2006-03-15 [PMID: 16517749] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CCL24
• Uniprot:
  • AAB51135()