Human CCL8/MCP-2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL8/MCP-2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL8/MCP-2 DuoSet ELISA, 15 Plate

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP-2 is a CC chemokine subfamily monocyte chemotactic protein. MCP-2 also chemoattracts activated NK cells, CD4+ and CD8+ T lymphocytes, and eosinophils and can induce histamine secretion from basophils. MCP-2 shares 62% amino acid sequence identity with MCP-1 and 58% with MCP-3.

Publications for CCL8/MCP-2 (DY281)(13)

Publications using DY281

• He, J;Li, L;Xiong, D;Liu, F;An, J;Bai, W;Hu, Y;Liu, L;Liu, D; The ferroptosis-associated gene TIMP1 facilitates skin scar formation through the interaction with CST3 in fibroblasts International immunopharmacology 2025-09-08 [PMID: 40925202] (Human)
• Parween, F;Singh, SP;Kathuria, N;Zhang, HH;Ashida, S;Otaizo-Carrasquero, FA;Shamsaddini, A;Gardina, PJ;Ganesan, S;Kabat, J;Lorenzi, HA;Riley, DJ;Myers, TG;Pittaluga, S;Bielekova, B;Farber, JM; Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines Nature communications 2025-02-26 [PMID: 40000641] (Human)
• F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (Human)
• AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation PLoS Pathog., 2020-08-18;16(8):e1008781. 2020-08-18 [PMID: 32810179] (Human)
• Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016-07-26;6(0):24530. 2016-07-26 [PMID: 27456172] (Human)
• Pallister K, Mason S, Nygaard T, Liu B, Griffith S, Jones J, Linderman S, Hughes M, Erickson D, Voyich J, Davis M, Wilson E Bovine CCL28 Mediates Chemotaxis via CCR10 and Demonstrates Direct Antimicrobial Activity against Mastitis Causing Bacteria. PLoS ONE, 2015-09-11;10(9):e0138084. 2015-09-11 [PMID: 26359669] (Bovine)
• Navas A, Vargas D, Freudzon M, McMahon-Pratt D, Saravia N, Gomez M Chronicity of dermal leishmaniasis caused by Leishmania panamensis is associated with parasite-mediated induction of chemokine gene expression. Infect Immun, 2014-04-21;82(7):2872-80. 2014-04-21 [PMID: 24752514] (Human)
• Sierra-Filardi E, Nieto C, Dominguez-Soto A, Barroso R, Sanchez-Mateos P, Puig-Kroger A, Lopez-Bravo M, Joven J, Ardavin C, Rodriguez-Fernandez J, Sanchez-Torres C, Mellado M, Corbi A CCL2 shapes macrophage polarization by GM-CSF and M-CSF: identification of CCL2/CCR2-dependent gene expression profile. J Immunol, 2014-03-17;192(8):3858-67. 2014-03-17 [PMID: 24639350] (Human)
• Ho L, Hung L, Liu F, Hou T, Lin L, Huang C, Lai J Ginkgo biloba extract individually inhibits JNK activation and induces c-Jun degradation in human chondrocytes: potential therapeutics for osteoarthritis. PLoS ONE, 2013-12-11;8(12):e82033. 2013-12-11 [PMID: 24349175] (Human)
• Sakakibara S, Espigol-Frigole G, Gasperini P, Uldrick T, Yarchoan R, Tosato G A20/TNFAIP3 inhibits NF-kappaB activation induced by the Kaposi's sarcoma-associated herpesvirus vFLIP oncoprotein. Oncogene, 2012-04-23;32(10):1223-32. 2012-04-23 [PMID: 22525270] (Human)
• Ockinger J, Stridh P, Beyeen AD, Lundmark F, Seddighzadeh M, Oturai A, Sorensen PS, Lorentzen AR, Celius EG, Leppa V, Koivisto K, Tienari PJ, Alfredsson L, Padyukov L, Hillert J, Kockum I, Jagodic M, Olsson T Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis. Genes Immun., 2009-10-29;11(2):142-54. 2009-10-29 [PMID: 19865101] (Human)
• Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function. Blood, 2006-04-13;108(3):947-55. 2006-04-13 [PMID: 16614246] (Human)
• Pierer M, Rethage J, Seibl R, Lauener R, Brentano F, Wagner U, Hantzschel H, Michel BA, Gay RE, Gay S, Kyburz D Chemokine secretion of rheumatoid arthritis synovial fibroblasts stimulated by Toll-like receptor 2 ligands. J. Immunol., 2004-01-15;172(2):1256-65. 2004-01-15 [PMID: 14707104] (Human)

FAQs for CCL8/MCP-2 (DY281). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL8