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Canine CCL20/MIP-3 alpha - Ready-To-Use ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
15.6 - 1000 pg/mL (example only; lot dependent)
Sensitivity
6.1 pg/mL (example only; lot dependent)

Order Details

Canine CCL20/MIP-3 alpha - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
15.6 - 1000 pg/mL (example only; lot dependent)
Sensitivity
6.1 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL20

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Alternate Names for Canine CCL20/MIP-3 alpha - Ready-To-Use ELISA Kit (Colorimetric)

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibitsproliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of themucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processedforms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coliATCC 25922 and S.aureus ATCC 29213

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CCL20/MIP-3 alpha ELISA Kit (NBP3-55515). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol CCL20