Human CCL20/MIP-3 alpha ELISA Kit (Colorimetric)

ELISA: Human CCL20/MIP-3 alpha ELISA Kit (Colorimetric) [NBP2-31049] - Standard curve using the CCL20/MIP-3 alpha standard.

Product Discontinued

Human CCL20/MIP-3 alpha ELISA Kit (Colorimetric) Summary

• Description: Human CCL20/MIP-3alpha ActivELISA
• Specificity: Measures free MIP-3 alpha in the supernatant of activated cells.
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 4 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CCL20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the Detection of Human CCL20/MIP-3 alpha in the Supernatant. The human MIP-3 alpha ActivELISA(TM) Kit measures free MIP-3 alpha in the supernatant of activated cells. Standard protocols for detecting MIP-3 alpha activity include ELISA and Western Blot. The MIP-3 alpha ActivELISA(TM) can be completed in one day using a sandwich ELISA protocol. The anti-MIP-3 alpha antibody is coated on a plate which then captures free MIP-3 alpha. The amount of bound MIP3-alpha is detected by adding a second biotinylated anti-MIP-3 alpha antibody followed by HRP-conjugated streptavidin. ABTS substrate is then added and the concentration is determined by colorimetric detection in an ELISA plate reader.

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

Additional items required for the ELISA (not included in the MIP-3 alpha ActivELISA(TM) Module): 96-well ELISA plate reader.

Alternate Names for Human CCL20/MIP-3 alpha ELISA Kit (Colorimetric)

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20/MIP-3Alpha is a small cytokine of the CC chemokine family. It acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. It is expressed constitutively by keratinocytes in epidermal layers of the skin, intestinal mucosa, epithelial crypts of tonsils, and the epithelium of Payer's patches in the intestine (Hoover DM, et al, 2002). MIP-3Alpha triggers an adaptive immune response primarily by attracting immature dendritic cells expressing CCR6 on their surfaces to the site of inflammation, and allows the dendritic cell to take up a foreign antigen and to mature. After binding, CCR6 expression is down-regulated, causing the dendritic cell to migrate to the lymph nodes, where it binds via CCR7 and presents the antigen to CD8 T cells. Thus, it forms a bridge between innate and adaptive immune response (Serafini, et al, 2002). MIP-3Alpha plays an immense role in various cancer entities, such as leukemia, lymphoma, melanoma, hepatocellular carcinoma, prostate cancer, colorectal adenocarcinoma and lung and oral squamous cell carcinoma (Ghadjar P, et al, 2006, 2008). MIP-3Alpha along with its corresponding receptor CCR6 is significantly up-regulated in patients with pancreatic cancer. But inhibition of CCR6 signaling or neutralization of MIP-3Alpha or inhibition of its production and activity may be useful in preventing further progression of the disease and may be a future basic treatment strategy in the management of pancreatic cancer (Campbell AS, et al, 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CCL20/MIP-3 alpha ELISA Kit (NBP2-31049)(4)

Publications using NBP2-31049

• Zoller V, Funcke JB, Roos J et al. Trail (TNF-related apoptosis-inducing ligand) induces an inflammatory response in human adipocytes Sci Rep 2017-07-18 [PMID: 28720906] (ELISA, Human)
• Fuhr L, Rousseau M, Plauth A et al. Amorfrutins Are Natural PPARgamma Agonists with Potent Anti-inflammatory Properties. J. Nat. Prod. 2015-05-22 [PMID: 25938459] (ELISA, Human)
  
  Details:
  CCL20/MIP-3 alpha ActivELISA Kit was used for the quantification of MIP-3a/CCL20 in cell culture supernatants from HT-29 cells subjected or not to treatments with TNF-alpha and/or Amorfrutin A, a phenolic constituents/Amorfrutins derived from Amorpha fruticosa (desert false indigo/ bastard indigobush). See full text for experimental details and Figure 3A for the ELISA results.
• Vossenkamper A, Marches O, Fairclough PD et al. Inhibition of NF-kB signaling in human dendritic cells by the enteropathogenic Escherichia coli effector protein NleE. J Immunol. 2010-10-01 [PMID: 20833837] (Human)
  
  Details:
  Nuclear and cytosolic extracts from monocyte derived human dendritic cells in the presence of EPEC LPS, Fig 3D.
• Kondo A, Yamashita T, Tamura H et al. Interferon-gamma and tumor necrosis factor-alpha induce an immunoinhibitory molecule, B7-H1, via nuclear factor-kappaB activation in blasts in myelodysplastic syndromes. Blood. 2010-08-19 [PMID: 20472834]
  
  Details:
  NF-kB/p65 ActivELISA (IMK-503) kit and Nuclear extraction kit (10081K). SKM-1 leukemic cells were separated into cytoplasmic and nuclear fractions then subject to western blot analysis, Fig 2A &B.

FAQs for CCL20/MIP-3 alpha ELISA Kit (NBP2-31049). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: CCL20