Western Blot: CCL20/MIP-3 alpha Antibody [NBP1-42763] - Analysis of a band 26 kDa in size corresponding to recom-binant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight ...read more
Western Blot: CCL20/MIP-3 alpha Antibody [NBP1-42763] - Analysis using the Biotin conjugate of NBP1-42763. Detection of Lane 1: Human MIP-3a. Detection of Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 ...read more
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3a protein.
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
From 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
0.01% Sodium Azide
Reconstitute with 0.1 ml distilled water to a final concentration of 1.0 mg/ml.
The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 11 kDa in size corresponding to native human MIP-3a protein is expected in the appropriate cell lysate or extract.
Read Publication using NBP1-42763 in the following applications:
small inducible cytokine subfamily A (Cys-Cys), member 20
MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.