CCL20/MIP-3 alpha Antibody

Western Blot: CCL20/MIP-3 alpha Antibody [NBP1-42763] - Analysis of a band 26 kDa in size corresponding to recombinant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.

Western Blot: CCL20/MIP-3 alpha Antibody [NBP1-42763] - Analysis using the Biotin conjugate of NBP1-42763. Detection of Lane 1: Human MIP-3a. Detection of Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 for 60 min at RT.

Product Discontinued

CCL20/MIP-3 alpha Antibody Summary

• Description: CCL20/MIP-3 alpha Antibody was heated to 56C for 30 minutes.
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3 alpha protein.
• Specificity: In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCL20
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:20000-1:100000
  • Western Blot 1:500-1:2000
• Application Notes: The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 11 kDa in size corresponding to native human MIP-3a protein is expected in the appropriate cell lysate or extract.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water.

Alternate Names for CCL20/MIP-3 alpha Antibody

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL20/MIP-3 alpha Antibody (NBP1-42763)(1)

Publication using NBP1-42763

• Ho MF, Bongartz T, Liu M et al. Estrogen, SNP-Dependent Chemokine Expression and Selective Estrogen Receptor Modulator Regulation. Mol. Endocrinol. 2016-03-01 [PMID: 26866883] (WB, Human)
  
  Details:
  B7-H4 antibody was used at a dilution of 1:400 for the IHC-P analysis of tissues from ovarian cancer patients (serous cystadenocarcinoma and mucinous cystadenocarcinoma). The detection was performed using HRP labelled secondary and DAB reagent - see the full text for detailed protocol.

Bioinformatics

• Gene Symbol: CCL20
• Entrez:
  • 6364(Human)
• Uniprot:
  • P78556()