CCL2/MCP1 Antibody (2D8) - Azide and BSA Free

Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated (vehicle control, e), 1uM lapatinib (f) or 1 uM IKKi compound A (g) for 24 hrs. Bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02481-5), licensed under a CC-BY license. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining. Image from the standard format of this antibody.

Flow Cytometry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.

Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa) Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). B) Staining without MCP1 mAb Image from the standard format of this antibody.

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, CCR2 signal, zoom factor 1x; full image in Supplementary Fig. 5i. CCL2 intensity was quantified usin

ELISA: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Fe, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CCL2/MCP1 Antibody (2D8) - Azide and BSA Free Summary

• Immunogen: Full lenght human MCP1 expressed in E. coli. [Uniprot: P13500]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29286133).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CCL2/MCP1 Antibody (2D8) - Azide and BSA Free

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL2