Biological Strategies: Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated ...read more
Immunohistochemistry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, ...read more
Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa ...read more
Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates.
Flow Cytometry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.
11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (PMID: 29286133).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein A or G purified
Alternate Names for CCL2/MCP1 Antibody (2D8)
C-C motif chemokine 2
chemokine (C-C motif) ligand 2
HC11Monocyte secretory protein JE
MCAFMonocyte chemotactic and activating factor
MCP1 Monocyte chemotactic protein 1
MCP-1 Small-inducible cytokine A2
Monocyte chemoattractant protein 1
monocyte chemoattractant protein-1
small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
small inducible cytokine subfamily A (Cys-Cys), member 2
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Heat-mediated Antigen Retrieval with 10mM Na Citrate buffer, pH6.0. Block with 5% Normal Goat Serum in PBS for 30min, RT Primary antibody (MCP-1, 1:500 dilution) overnight, 4 C HRP-conjugated 2nd antibody (1:500) for 30min, RT DAB staining Countersigning with hematoxylin
Product General Protocols
View specific protocols for CCL2/MCP1 Antibody (NBP2-22115):
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