CCL2/MCP1 Antibody (2D8)

Images

 
Biological Strategies: Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated ...read more
Immunohistochemistry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, ...read more
Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa ...read more
Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates.
Flow Cytometry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.
ELISA: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Mu, Rt, Fe, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

CCL2/MCP1 Antibody (2D8) Summary

Immunogen
Full lenght human MCP1 expressed in E. coli. [Uniprot: P13500]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CCL2/MCP1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP2-22115 in the following applications:

Publications
Read Publications using
NBP2-22115 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29286133).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CCL2/MCP1 Antibody (2D8)

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL2/MCP1 Antibody (NBP2-22115)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(1)
ICC/IF
(3)
IHC
(1)
IHC-P
(4)
WB
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(6)
Rat
(1)
Feline
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP2-22115 Applications Species
Sorrentino G, Rezakhani S, Yildiz E et al. Mechano-modulatory synthetic niches for liver organoid derivation Nat Commun Jul 10 2020 [PMID: 32651372] (WB, Human, Mouse) WB Human, Mouse
Upmanyu N, Bulldan A, Failing K, Scheiner-Bobis G DHEAS prevents pro-metastatic and proliferative effects of 17 beta-estradiol on MCF-7 breast cancer cells Biochim Biophys Acta Mol Cell Res Nov 21 2019 [PMID: 31760088] (ICC/IF, Human) ICC/IF Human
Wenstedt EF, Verberk SG, Kroon J, et al. Salt increases monocyte CCR2 expression and inflammatory responses in humans JCI Insight 2019 Nov 01 [PMID: 31672939] (IHC-P, Human) IHC-P Human
Pascual-Garcia M, Bonfill-Teixidor E, Planas-Rigol E et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy Nat Commun Jun 11 2019 [PMID: 31186412] (IHC, ICC/IF, Human, Mouse) IHC, ICC/IF Human, Mouse
Do C, Drel V, Tan C, Lee D et al. Nephrogenic' systemic fibrosis is mediated by myeloid C-C chemokine receptor 2 J. Invest. Dermatol. Apr 9 2019 [PMID: 30978353]
Rojo JL, Jaworski JP, Peluffo MC. Direct role of the C-C motif chemokine receptor 2/monocyte chemoattractant protein 1 system in the feline cumulus oocyte complex. Biol. Reprod. Nov 3 2018 [PMID: 30395163] (ICC/IF, Feline) ICC/IF Feline
Beke G, Dajnoki Z, Kapitany A et al. Immunotopographical Differences of Human Skin. Front Immunol. Mar 5 2018 [PMID: 29556238] (IHC-P, Human) IHC-P Human
Garzia L, Kijima N, Morrissy AS, De Antonellis P. A Hematogenous Route for Medulloblastoma Leptomeningeal Metastases. Cell. 2018 Feb 22 [PMID: 29474906] (Flow, Human) Flow Human
Linde N, Casanova-Acebes M, Sosa MS et al. Macrophages orchestrate breast cancer early dissemination and metastasis Nat Commun 2018 Jan 02 [PMID: 29295986] (IHC-P, Mouse) IHC-P Mouse
Zhi LQ, Yao SX, Liu HL et al. Hydroxytyrosol inhibits the inflammatory response of osteoarthritis chondrocytes via SIRT6-mediated autophagy. Mol Med Rep 2018 Mar [PMID: 29286133] (Rat) Rat
Show All 14 Publications.

Review for CCL2/MCP1 Antibody (NBP2-22115) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rhesus macaque.

Reviews using NBP2-22115:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Rhesus macaque
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CCL2/MCP1 NBP2-22115
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reviewed by:
Anonymous
IHC-P Rhesus macaque 11/25/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHIV-infected Rhesus macaque adult lung
SpeciesRhesus macaque
LotA-7

Comments

CommentsHeat-mediated Antigen Retrieval with 10mM Na Citrate buffer, pH6.0.
Block with 5% Normal Goat Serum in PBS for 30min, RT
Primary antibody (MCP-1, 1:500 dilution) overnight, 4 C
HRP-conjugated 2nd antibody (1:500) for 30min, RT
DAB staining
Countersigning with hematoxylin

Product General Protocols

View specific protocols for CCL2/MCP1 Antibody (NBP2-22115): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCL2/MCP1 Antibody (NBP2-22115) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22115AF350
Alexa Fluor 405 NBP2-22115AF405
Alexa Fluor 488 NBP2-22115AF488
Alexa Fluor 532 NBP2-22115AF532
Alexa Fluor 594 NBP2-22115AF594
Alexa Fluor 647 NBP2-22115AF647
Alexa Fluor 700 NBP2-22115AF700
Alexa Fluor 750 NBP2-22115AF750
Allophycocyanin NBP2-22115APC
Biotin NBP2-22115B
DyLight 350 NBP2-22115UV
DyLight 405 NBP2-22115V
DyLight 488 NBP2-22115G
DyLight 550 NBP2-22115R
DyLight 594 NBP2-22115DL594
DyLight 650 NBP2-22115C
DyLight 680 NBP2-22115FR
DyLight 755 NBP2-22115IR
FITC NBP2-22115F
HRP NBP2-22115H
Janelia Fluor 549 NBP2-22115JF549
Janelia Fluor 646 NBP2-22115JF646
PE NBP2-22115PE
PerCP NBP2-22115PCP

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Bioinformatics Tool for CCL2/MCP1 Antibody (NBP2-22115)

Discover related pathways, diseases and genes to CCL2/MCP1 Antibody (NBP2-22115). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Recent Reviews

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5
0
4
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1
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0
1
0

Anonymous
11/25/2019
Application: IHC-P
Species: Rhesus macaque

Bioinformatics

Gene Symbol CCL2
Entrez
Uniprot