CCL19/MIP-3 beta Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC206523 and based on accession number NM_006274. The protein contains a C-terminal DDK tag.
Homo sapiens chemokine (C-C motif) ligand 19 (CCL19), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for CCL19/MIP-3 beta
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CCL19/MIP-3 beta Overexpression Lysate (Native)
- beta chemokine exodus-3
- Beta-chemokine exodus-3
- CC chemokine ligand 19
- C-C motif chemokine 19
- chemokine (C-C motif) ligand 19
- EBI1-ligand chemokine
- Epstein-Barr virus-induced molecule 1 ligand chemokine
- Macrophage inflammatory protein 3 beta
- macrophage inflammatory protein 3-beta
- MIP3 beta
- MIP-3 beta
- MIP3BCK beta-11
- SCYA19EBI1 ligand chemokine
- small inducible cytokine subfamily A (Cys-Cys), member 19
- Small-inducible cytokine A19
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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