CCL18/PARC Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det), Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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CCL18/PARC Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774
Specificity
Detects human CCL18/PARC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, rhMPIF‑1, rhMIP‑1 alpha, recombinant mouse (rm) MIP‑1 alpha, rhMIP‑1 beta, rmMIP‑1 beta, recombinant viral (rv) MIP‑I, rvMIP‑II, and rhMIP‑1δ is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL18
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human CCL18/PARC Antibody (Catalog # MAB394)
ELISA Detection: Human CCL18/PARC Biotinylated Antibody (Catalog # BAF394)
Standard: Recombinant Human CCL18/PARC (Catalog # 394-PA)
Readout System
Reviewed Applications
Read 4 Reviews rated 4.3
using
BAF394 in the following applications:

Publications
Read Publications using
BAF394 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL18/PARC Antibody [Biotin]

  • Alternative macrophage activation-associated CC chemokine 1
  • AMAC-1
  • AMAC1CC chemokine ligand 18
  • AMAC-1Small-inducible cytokine A18
  • CCL18
  • chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
  • CKb7
  • DC-CK1
  • DC-CK1CC chemokine PARC
  • DCCK1Macrophage inflammatory protein 4
  • Dendritic cell chemokine 1
  • MIP4
  • MIP-4
  • MIP-4C-C motif chemokine 18
  • PARC
  • PARCPulmonary and activation-regulated chemokine
  • SCYA18chemokine (C-C), dendritic
  • small inducible cytokine A18
  • small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.
  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL18/PARC Antibody (BAF394)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA detection, ICC, Luminex Development, Other: See notes.


Filter By Application
ELISA detection
(1)
ICC
(1)
Luminex Development
(1)
Other: See notes
(1)
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Filter By Species
Human
(4)
All Species

Reviews for CCL18/PARC Antibody (BAF394) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using BAF394:
Filter by Applications
ELISA
(4)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 01/14/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID: 19120345
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 19120345

Details

Detection NotesSee PMID: 19120345

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 19120345
 
reviewed by:
Anonymous
ELISA Human 01/14/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID: 21801246
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 21801246

Details

Detection NotesSee PMID: 21801246

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 21801246
 
reviewed by:
Anonymous
ELISA Human 01/14/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID: 19175890
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 19175890

Details

Detection NotesSee PMID: 19175890

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 19175890
 
reviewed by:
Anonymous
ELISA Human 01/13/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22280057
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22280057

Details

Detection NotesSee PMID 22280057

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22280057

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

 
Anonymous
01/14/2015
Application: ELISA
Species: Human

 
Anonymous
01/14/2015
Application: ELISA
Species: Human

 
Anonymous
01/14/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CCL18
Uniprot