CCL18/PARC Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL18/PARC Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL18/PARC
  Ala21-Ala89
  Accession # P55774
• Specificity: Detects human CCL18/PARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant mouse MIP-1 alpha is observed and less than 10% cross-reactivity with rhMPIF‑1, recombinant viral (rv) MIP‑I, rvMIP‑II, rhHCC-1, and rhMIP-1δ is observed.
• Details of Functionality: ActivityAssaywCitation not found None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL18

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL18/PARC Antibody [Unconjugated]

• Alternative macrophage activation-associated CC chemokine 1
• AMAC-1
• AMAC1CC chemokine ligand 18
• AMAC-1Small-inducible cytokine A18
• CCL18
• chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• CKb7
• DC-CK1
• DC-CK1CC chemokine PARC
• DCCK1Macrophage inflammatory protein 4
• Dendritic cell chemokine 1
• MIP4
• MIP-4
• MIP-4C-C motif chemokine 18
• PARC
• PARCPulmonary and activation-regulated chemokine
• SCYA18chemokine (C-C), dendritic
• small inducible cytokine A18
• small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

1. Adema, G. et al. (1997) Nature 387:713.
2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL18/PARC Antibody (AF394)(5)

Publications using AF394

• Momohara S, Okamoto H, Iwamoto T, Mizumura T, Ikari K, Kawaguchi Y, Takeuchi M, Kamatani N, Tomatsu T High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. J. Rheumatol., 2007;34(2):266-71. 2007 [PMID: 17304652] (IHC-P, Human)
• Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production. J. Allergy Clin. Immunol., 2006;118(2):354-60. 2006 [PMID: 16890758] (Neutralization, Human)
• Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18. Arthritis Rheum., 2006;54(8):2643-55. 2006 [PMID: 16868995] (Western Blot, Human)
• Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells. J. Immunol., 2005;174(3):1723-8. 2005 [PMID: 15661937] (IHC, Human)
• Pivarcsi A, Gombert M, Dieu-Nosjean MC, Kubitza R, Meller S, Rieker J, Muller A, Da Cunha L, Haahtela A, Sonkoly E, Fridman WH, Alenius H, Kemeny L, Ruzicka T, Zlotnik A CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure. J. Immunol., 2004;173(9):5810-7. 2004 [PMID: 15494534] (IHC, Human)

Review for CCL18/PARC Antibody (AF394) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous WB Other 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23874339
• Species: Other

Blocking

• Blocking Details: See PMID 23874339

Details

• Detection Notes: See PMID 23874339

Comments

• Comments: See PMID 23874339Independent review from antibodyresource.com"DESCRIPTION: Human CCL18/PARC Affinity Purified Polyclonal Ab, Goat IgG ANTIBODY APPLICATION: Western Blot and Flow cytometryCATALOG NUMBER: AF394"

Bioinformatics

• Gene Symbol: CCL18
• Uniprot:
  • P55774()