CCL18/PARC Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

CCL18/PARC Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human CCL18/PARC
  Ala21-Ala89
  Accession # P55774
• Specificity: Detects human CCL18/PARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant mouse MIP-1 alpha is observed and less than 10% cross-reactivity with rhMPIF‑1, recom
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL18/PARC Antibody [Alexa Fluor® 647]

• Alternative macrophage activation-associated CC chemokine 1
• AMAC-1
• AMAC1CC chemokine ligand 18
• AMAC-1Small-inducible cytokine A18
• CCL18
• chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• CKb7
• DC-CK1
• DC-CK1CC chemokine PARC
• DCCK1Macrophage inflammatory protein 4
• Dendritic cell chemokine 1
• MIP4
• MIP-4
• MIP-4C-C motif chemokine 18
• PARC
• PARCPulmonary and activation-regulated chemokine
• SCYA18chemokine (C-C), dendritic
• small inducible cytokine A18
• small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma . CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.