CCL17/TARC Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL17/TARC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
  Ala24-Ser94
  Accession # Q92583
• Specificity: Detects human CCL17/TARC in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant mouse TARC, recombinant human (rh) MIP-1 alpha , rhMIP-1 beta , rhRANTES, rhMCP-1, rhTECK, and rh6Ckine is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CCL17/TARC Antibody (Catalog # MAB364) ELISA Detection: Human CCL17/TARC Biotinylated Antibody (Catalog # BAF364) Standard: Recombinant Human CCL17/TARC (Catalog # 364-DN)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL17/TARC Antibody [Biotin]

• ABCD-2
• CC chemokine TARC
• C-C motif chemokine 17
• CCL17
• chemokine (C-C motif) ligand 17
• MGC138273
• SCYA17
• SCYA17MGC138271
• small inducible cytokine subfamily A (Cys-Cys), member 17
• Small-inducible cytokine A17
• T cell-directed CC chemokine
• TARC
• TARCA-152E5.3
• Thymus and activation-regulated chemokine

Background

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha , MIP-1 beta , MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
3. Nomiyama, H. et al. (1997) Genomics 40:211.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL17/TARC Antibody (BAF364)(9)

Publications using BAF364

• Johanna Huoman, Georgia Papapavlou, Anna Pap, Johan Alm, Lennart J. Nilsson, Maria C. Jenmalm Sublingual immunotherapy alters salivary IgA and systemic immune mediators in timothy allergic children Pediatric Allergy and Immunology 4/14/2019 [PMID: 30803044]
• T Kawahara, K Tsutsui, E Nakanishi, T Inoue, Y Hamauzu Effect of the topical application of an ethanol extract of quince seeds on the development of atopic dermatitis-like symptoms in NC/Nga mice BMC Complement Altern Med, 2017-01-31;17(1):80. 2017-01-31 [PMID: 28137259] (ELISA Development, Human)
• Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al. Placental immune response to apple allergen in allergic mothers Journal of Reproductive Immunology 12/1/2014 [PMID: 25048667]
• Paantjens AW, van de Graaf EA, Kwakkel-van Erp JM, Hoefnagel T, van Kessel DA, van den Bosch JM, Otten HG Lung transplantation affects expression of the chemokine receptor type 4 on specific T cell subsets. Clin. Exp. Immunol., 2011-10-01;166(1):103-9. 2011-10-01 [PMID: 21910727] (ELISA Development, Human)
• T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization Clinical & Experimental Allergy 12/1/2011 [PMID: 21801246]
• Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation Pediatric Research 3/1/2011 [PMID: 21099447]
• Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. J. Immunol., 2007-10-01;179(7):4840-8. 2007-10-01 [PMID: 17878383] (ELISA Development, Human)
• Liu L, Jarjour NN, Busse WW, Kelly EA Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24. 2004-03-04 [PMID: 15001464] (ELISA Development, Human)
• Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios. J. Immunol., 2001-06-01;166(11):6899-906. 2001-06-01 [PMID: 11359851] (ELISA Development, Human)

Review for CCL17/TARC Antibody (BAF364) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 01/13/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 21801246
• Species: Human

Bioinformatics

• Gene Symbol: CCL17
• Uniprot:
  • Q92583()