CCL16/HCC-4/LEC Antibody [Unconjugated]

Recombinant Human CCL16/HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/HCC‑4 (750 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL16/HCC‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF802). The ND50 is typically 4‑16 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL16/HCC-4/LEC Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL16/HCC-4
  Gln24-Gln120
  Accession # O15467
• Specificity: Detects human CCL16/HCC‑4 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL16
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 4-16 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL16/HCC-4/LEC Antibody [Unconjugated]

• C-C motif chemokine 16
• CCL16
• chemokine (C-C motif) ligand 16
• Chemokine CC-4
• Chemokine LEC
• CKb12
• HCC4
• HCC-4
• HCC-4Liver-expressed chemokine
• LCC-1Lymphocyte and monocyte chemoattractant
• LEC
• liver CC chemokine-1
• LMC
• LMCSmall-inducible cytokine A16
• monotactin-1
• Mtn-1
• NCC-4
• NCC4IL-10-inducible chemokine
• NCC-4ILINCK
• new CC chemokine 4
• SCYA16MGC117051
• SCYL4
• small inducible cytokine subfamily A (Cys-Cys), member 16

Background

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.

Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

1. Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
2. Hedrick, J. et al. (1998) Blood 91:4242.
3. Youn, B-S. et al. (1998) BBRC 247:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL16/HCC-4/LEC Antibody (AF802)(2)

Publications using AF802

• Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human)
• Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2005-08-17;65(3):294-300. 2005-08-17 [PMID: 16107514] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: CCL16
• Uniprot:
  • O15467()