CCL14a/HCC-1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL14a/HCC-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL14a/HCC-1 (R&D Systems, Catalog # 324-HC)
  Thr20-Asn93
  Accession # Q16627
• Specificity: Detects human CCL14a/HCC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) CCL16, rhCCL8, rhCCL7, rhCCL4, and rhCCL3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL14
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CCL14/HCC-1/HCC-3 Antibody (Catalog # MAB3241)
  ELISA Detection: Human CCL14a/HCC-1 Biotinylated Antibody (Catalog # BAF324)
  Standard: Recombinant Human CCL14a/HCC-1 aa 20-93 (Catalog # 324-HC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14a/HCC-1 Antibody [Biotin]

• C-C motif chemokine 14;CC-1;CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;MCIF;NCC2;NCC-2;SCYA14;SCYL2;SY14
• CCL14
• CCL14a
• HCC1
• HCC-1

Background

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue  precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20 - 93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

1. Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295.
2. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357.
3. Richter, R. et al. (2000) Biochemistry 39:10799.
4. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982.
5. Vakili, J. et al. (2001) J. Immunol. 167:3406.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL14a/HCC-1 Antibody (BAF324)(1)

Publication using BAF324

• Savino B, Borroni EM, Torres NM, Proost P, Struyf S, Mortier A, Mantovani A, Locati M, Bonecchi R Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence. J. Biol. Chem., 2009-07-24;284(38):26207-15. 2009-07-24 [PMID: 19632987] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CCL14
• Uniprot:
  • Q16627()