CCL14a/HCC-1 Products

1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL14a/ HCC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-324-PB). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_lightbox" target="_blank">

324-HC) was serially diluted and captured by Rat Anti-Human CCL14/HCC‑1/HCC‑3 Monoclonal Antibody (Catalog # MAB3241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL14a/HCC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-324-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">