CCL14a/HCC-1 Antibody

Recombinant Human CCL14a/HCC‑1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL14a/HCC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-324-PB). The ND50 is typically 0.15‑0.75 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL14a/HCC-1 Antibody Summary

• Immunogen: E. coli-derived recombinant human CCL14a/HCC‑1
  Thr20-Asn93
  Accession # Q16627
• Specificity: Detects human CCL14a/HCC-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human (rh) MCP-2, rhMCP-3, recombinant mouse (rm) C10, and rmMIP-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL14
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.15-0.75 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14a/HCC-1 Antibody

• C-C motif chemokine 14;CC-1;CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;MCIF;NCC2;NCC-2;SCYA14;SCYL2;SY14
• CCL14
• CCL14a
• HCC1
• HCC-1

Background

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20-93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC-1/CCL14a (aa 28-93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28-93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

1. Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295.
2. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357.
3. Richter, R. et al. (2000) Biochemistry 39:10799.
4. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982.
5. Vakili, J. et al. (2001) J. Immunol. 167:3406.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL14
• Uniprot:
  • Q16627()