Recombinant Human CCL11/Eotaxin Protein

Product Discontinued

Recombinant Human CCL11/Eotaxin Protein Summary

• Description: Recombinant bioactive protein containing 74 amino acids for Human CCL11/Eotaxin
  
  

  Source:E. coli
  Amino Acid Sequence:(P51671)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: The ED50 of human Eotaxin by chemotaxis based assays is 10 ng/ml using human peripheral blood eosinophils.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL11
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 20 mM phosphate buffer (pH 7.4), 150 mM NaCl.
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstituted in water to a concentration of 0.1 - 1 ug/ul. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Alternate Names for Recombinant Human CCL11/Eotaxin Protein

• C-C motif chemokine 11
• CCL11
• chemokine (C-C motif) ligand 11
• Eosinophil chemotactic protein
• Eotaxin
• eotaxin-1
• MGC22554
• SCYA11Small-inducible cytokine A11
• small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

Eotaxin is a recently discovered chemokine characterized by its high chemotactic selectivity for eosinophils. The position of the four cystein residues in Eotaxin places it in the C-C family of chemokines, along with chemokines such as RANTES, MCP-3, and MIP-1 alpha. The human Eotaxin is an 8.3 kDa protein containing 74 amino acid residues. The sequence of the first five N-terminal amino acids is Gly-Pro-Ala-Ser-Val.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL11