CCL11/Eotaxin Antibody [Unconjugated]

Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL11/Eotaxin (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA). The ND50 is typically 0.5-2.5 µg/mL.

CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human skin using Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑320‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL11/Eotaxin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL11/Eotaxin
  Gly24-Pro97
  Accession # Q6I9T4
• Specificity: Detects human CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse CCL11/Eotaxin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL11
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL11/Eotaxin Antibody [Unconjugated]

• C-C motif chemokine 11
• CCL11
• chemokine (C-C motif) ligand 11
• Eosinophil chemotactic protein
• Eotaxin
• eotaxin-1
• MGC22554
• SCYA11Small-inducible cytokine A11
• small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL11/Eotaxin Antibody (AF-320-NA)(2)

Publications using AF-320-NA

• Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities. J. Immunol., 2006-03-15;176(6):3788-95. 2006-03-15 [PMID: 16517749] (Western Blot, Human)
• Sato E, Koyama S Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity. Eur. Respir. J., 2000-11-01;16(5):951-8. 2000-11-01 [PMID: 11153598] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CCL11
• Uniprot:
  • Q6I9T4()