CCL1/I-309/TCA-3 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL1/I-309/TCA-3 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3
  Lys24-Cys92
  Accession # P10146
• Specificity: Detects mouse CCL1/I-309/TCA-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human I-309, recombinant mouse (rm) Eotaxin-2, rmTARC, and rmMIP-3 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CCL1/I-309/TCA-3 Antibody (Catalog # MAB8451)
  ELISA Detection: Mouse CCL1/I-309/TCA-3 Biotinylated Antibody (Catalog # BAF845)
  Standard: Recombinant Mouse CCL1/I-309/TCA-3 (Catalog # 845-TC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL1/I-309/TCA-3 Antibody [Biotin]

• C-C motif chemokine 1
• CCL1
• chemokine (C-C motif) ligand 1
• I-309
• inflammatory cytokine I-309
• P500
• SCYA1
• SCYA1Small-inducible cytokine A1
• SCYA2
• SISe
• small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• T lymphocyte-secreted protein I-309
• TCA3
• TCA-3

Background

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha , MIP-1 beta , and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

1. Goya, I. et al. (1998) J. Immunol. 160:1975.
2. Burd, P. et al. (1987) J. Immunol. 139:3126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL1/I-309/TCA-3 Antibody (BAF845)(5)

Publications using BAF845

• Caroline L. Sokol, Ryan B. Camire, Michael C. Jones, Andrew D. Luster The chemokine receptor CCR8 promotes the migration of dendritic cells into the lymph node parenchyma to initiate the allergic immune response. Immunity 9/1/2018 [PMID: 30170811]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA Development, Mouse)
• Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA Development, Mouse)
• Mosmann T Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Eur. J. Immunol., 2004-06-01;34(6):1617-26. 2004-06-01 [PMID: 15162431] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL1
• Uniprot:
  • P10146()