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845-TC) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/ TCA-3 (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CCL1/TCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF845). The ND50 is typically 0.3-1.5 µg/mL." class="big_lightbox" target="_blank">
845-TC) was serially diluted and captured by Rat Anti-Mouse CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB8451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL1/I‑309/TCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF845) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
845-TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA‑3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL." class="big_lightbox" target="_blank">
272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I-309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically 0.1-0.5 µg/mL." class="big_lightbox" target="_blank">
272-I) was serially diluted and captured by Mouse Anti-Human CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB272) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL1/I‑309/TCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
SCYA1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8. [provided by RefSeq]