CBX3 Antibody

Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CBX3 Antibody Summary

• Description: Novus Biologicals Goat CBX3 Antibody (NB100-2418) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-CBX3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD corresponding to internal region according to NP_057671.2.
• Specificity: No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2 - 3 ug/mL
  • Immunohistochemistry-Paraffin 2 - 3 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Control:

  CBX3 Overexpression Lysate
  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBX3 Antibody

• chromobox homolog 3 (Drosophila HP1 gamma)
• chromobox homolog 3
• chromobox protein homolog 3
• Drosophila)
• HECH
• Heterochromatin protein 1 homolog gamma
• heterochromatin protein HP1 gamma
• heterochromatin-like protein 1
• HP1 gamma homolog
• HP1 gamma
• HP1-GAMMA
• Modifier 2 protein

Background

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBX3 Antibody (NB100-2418)(1)

Publication using NB100-2418

• Vakoc CR, Mandat SA, Olenchock BA, Blobel GA. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Mol Cell 2005-08-05 [PMID: 16061184]

Bioinformatics

• Gene Symbol: CBX3
• Entrez:
  • 11335(Human)
  • 12417(Mouse)
  • 297093(Rat)
• Uniprot:
  • NP_009207.2(Human)