CBX3 Antibody (1G12-1D9) - Azide and BSA Free

Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.

Immunocytochemistry/Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Analysis of monoclonal antibody to CBX3 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Detection limit for recombinant GST tagged CBX3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1G12-1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CBX3 Antibody (1G12-1D9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CBX3 Antibody (1G12-1D9) - Azide and BSA Free (H00011335-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-CBX3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CBX3 (AAH00954, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMLLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ
• Specificity: CBX3 - chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBX3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CBX3 Antibody (1G12-1D9) - Azide and BSA Free

• chromobox homolog 3 (Drosophila HP1 gamma)
• chromobox homolog 3
• chromobox protein homolog 3
• Drosophila)
• HECH
• Heterochromatin protein 1 homolog gamma
• heterochromatin protein HP1 gamma
• heterochromatin-like protein 1
• HP1 gamma homolog
• HP1 gamma
• HP1-GAMMA
• Modifier 2 protein

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBX3 Antibody (H00011335-M01)(2)

Publications using H00011335-M01

• Hsieh MY, Fan JR, Chang HW et al. DNA Topoisomerase III Alpha Regulates p53-Mediated Tumor Suppression. Clin Cancer Res 2014-02-13 [PMID: 24526736]
• Lin TY, Cheng YC, Yang HC et al. Loss of the candidate tumor suppressor BTG3 triggers acute cellular senescence via the ERK-JMJD3-p16(INK4a) signaling axis. Oncogene. 2012-07-05 [PMID: 22020331] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CBX3
• Entrez:
  • 11335(Human)
• OMIM:
  • 604477(Human)
• Uniprot:
  • AAH00954(Human)