Caveolin-2 Antibody [Biotin]

Caveolin‑2 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL overnight at 4° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Caveolin‑2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Caveolin-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Caveolin-2
  Met1-Lys86
  Accession # P51636
• Specificity: Caveolin-2 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse, and rat Caveolin-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CAV2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caveolin-2 Antibody [Biotin]

• CAV
• CAV2
• caveolae protein, 20-kD
• caveolin 2 isoform a and b
• caveolin 2
• Caveolin2
• Caveolin-2
• MGC12294

Background

Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAV2
• Uniprot:
  • P51636()