Caveolin-2 Antibody [Unconjugated]

Western blot shows recombinant human Caveolin-1, Caveolin-2 and Caveolin-3 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin‑2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Caveolin-2 was detected in paraffin-embedded sections of human liver using Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin‑2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Caveolin-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Caveolin-2
  Met1-Lys86
  Accession # P51636
• Specificity: Detects endogenous human, mouse, and rat Caveolin-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CAV2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caveolin-2 Antibody [Unconjugated]

• CAV
• CAV2
• caveolae protein, 20-kD
• caveolin 2 isoform a and b
• caveolin 2
• Caveolin2
• Caveolin-2
• MGC12294

Background

Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caveolin-2 Antibody (AF5788)(2)

Publications using AF5788

• Sasamoto, Y;Kiritoshi, S;Lee, CAA;Fukuda, Y;Martin, G;Ksander, BR;Frank, MH;Frank, NY; Caveolin 1 and 2 enhance the proliferative capacity of BCAM-positive corneal progenitors Scientific reports 2025-02-24 [PMID: 39994275] (Immunohistochemistry, Human)
• M Mircea, M Hochane, X Fan, SM Chuva de S, D Garlaschel, S Semrau Phiclust: a clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations Genome Biology, 2022-01-10;23(1):18. 2022-01-10 [PMID: 35012604] (IHC, Human)

Bioinformatics

• Gene Symbol: CAV2
• Uniprot:
  • P51636()