Caveolin-1 Antibody (7C8) [Biotin]

Caveolin-1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Biotinylated Monoclonal Antibody (Catalog # BAM5736) at 25 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 493-conjugated Streptavidin (green; Catalog # NL997) and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF
• Clone: 7C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Caveolin-1 Antibody (7C8) [Biotin] Summary

• Immunogen: Purified rat adipocyte low density microsomes
  Accession # P41350
• Specificity: Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse and rat Caveolin-1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAV1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with BSA as a carrier protein.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caveolin-1 Antibody (7C8) [Biotin]

• CAV
• CAV1
• caveolae protein, 22kD
• caveolin 1, caveolae protein, 22kDa
• Caveolin1
• Caveolin-1
• cell growth-inhibiting protein 32
• MSTP085
• VIP21

Background

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (amino acids 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% amino acid sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAV1
• Uniprot:
  • P41350()