Caveolin-1 [p Tyr14] Antibody (750304)

HAF018). A specific band was detected for Phospho-Caveolin-1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF018). A specific band was detected for Phospho-Caveolin-1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Caveolin-1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Caveolin-1 phosphorylated at Y14 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Caveolin-1 phosphorylated at Y14 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 750304
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Caveolin-1 [p Tyr14] Antibody (750304) Summary

• Immunogen: Phosphopeptide containing the human Caveolin-1 Y14 site
  Accession # Q03135
• Modification: p Tyr14
• Specificity: Detects human Phospho-Caveolin-1 (Y14) in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAV1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caveolin-1 [p Tyr14] Antibody (750304)

• CAV
• CAV1
• caveolae protein, 22kD
• caveolin 1, caveolae protein, 22kDa
• Caveolin1
• Caveolin-1
• cell growth-inhibiting protein 32
• MSTP085
• VIP21

Background

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caveolin-1 Antibody (MAB7418)(1)

Publication using MAB7418

• T Tomono, K Yano, T Ogihara Snail-Induced Epithelial-to-Mesenchymal Transition Enhances P-gp-Mediated Multi Drug Resistance in HCC827 Cells J Pharm Sci, 2017-03-18;0(0):. 2017-03-18 [PMID: 28322937] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CAV1
• Uniprot:
  • Q03135()