Cathepsin X/Z/P Antibody [Unconjugated]

Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, IP, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin X/Z/P Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
  Ala23-Val306
  Accession # Q9WUU7
• Specificity: Detects mouse Cathepsin X/Z/P in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ctsz
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 2.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin X/Z/P Antibody [Unconjugated]

• Cathepsin P
• Cathepsin X/Z/P
• Cathepsin Z
• CTSX
• CTSZ

Background

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
3. Nagler and Menard (1998) FEBS Lett. 434:135.
4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
7. Menard, et al. (2001) Biol. Chem. 382:839.
8. Therrien, et al. (2001) Biochemistry 40:2702.
9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
10. Guncar, et al. (2000) Structure Fold Des. 8:305.
11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin X/Z/P Antibody (AF1033)(11)

Publications using AF1033

• E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022-08-17;13(1):4848. 2022-08-17 [PMID: 35977928] (Western Blot, Mouse)
• Mahmoud Bassal, Junling Liu, Wanda Jankowiak, Paul Saftig, Udo Bartsch Rapid and Progressive Loss of Multiple Retinal Cell Types in Cathepsin D-Deficient Mice—An Animal Model of CLN10 Disease † Cells 3/21/2021 [PMID: 33800998]
• Y Atiskova, S Bartsch, T Danyukova, E Becker, C Hagel, S Storch, U Bartsch Mice deficient in the lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) display a complex retinal phenotype Sci Rep, 2019-10-02;9(1):14185. 2019-10-02 [PMID: 31578378] (IHC-Fr, Western Blot, Mouse)
• DM Chopyk, P Kumar, R Raeman, Y Liu, T Smith, FA Anania Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers Physiol Rep, 2017-12-01;5(23):. 2017-12-01 [PMID: 29208693] (ICC, Western Blot, Human)
• Janko Kos Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation Neuropharmacology, 2016-11-23;114(0):88-100. 2016-11-23 [PMID: 27889490] (IHC, Western Blot, Mouse)
• Masayuki Suyama, Masato Koike, Daisuke Asaoka, Hiroki Mori, Masako Oguro, Takashi Ueno et al. Increased Immunoreactivity of Cathepsins in the Rat Esophagus under Chronic Acid Reflux Esophagitis Journal of Histochemistry & Cytochemistry 9/1/2014 [PMID: 24943348]
• Makrypidi G, Damme M, Muller-Loennies S, Trusch M, Schmidt B, Schluter H, Heeren J, Lubke T, Saftig P, Braulke T Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5. Mol. Cell. Biol., 2011-12-12;32(4):774-82. 2011-12-12 [PMID: 22158965] (Western Blot, Mouse)
• Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011-12-01;25(23):2465-79. 2011-12-01 [PMID: 22156207] (Western Blot, Mouse)
• Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin. Blood, 2011-07-27;118(12):3384-91. 2011-07-27 [PMID: 21795745] (Western Blot, Mouse)
• Margot G. Paulick, Matthew Bogyo Development of Activity-Based Probes for Cathepsin X ACS Chemical Biology 6/17/2011 [PMID: 21322635]
• Wendt W, Schulten R, Stichel CC, Lubbert H Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. J. Neurochem., 2009-07-17;110(6):1931-41. 2009-07-17 [PMID: 19627446] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ctsz
• Uniprot:
  • Q9WUU7()