Cathepsin X/Z/P Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Cathepsin X/Z/P Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
  Ala23-Val306
  Accession # Q9WUU7
• Specificity: Detects mouse Cathepsin X/Z/P in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin-D, rmCathepsin-H, rmCathepsin-L, rmCathepsin-C, and rmCathepsin-B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ctsz
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin X/Z/P Antibody [Biotin]

• Cathepsin P
• Cathepsin X/Z/P
• Cathepsin Z
• CTSX
• CTSZ

Background

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1-5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7-10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
3. Nagler and Menard (1998) FEBS Lett. 434:135.
4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
7. Menard, et al. (2001) Biol. Chem. 382:839.
8. Therrien, et al. (2001) Biochemistry 40:2702.
9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
10. Guncar, et al. (2000) Structure Fold Des. 8:305.
11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin X/Z/P Antibody (BAF1033)(2)

Publications using BAF1033

• Alejandro Gomez-Auli, Larissa Elisabeth Hillebrand, Daniel Christen, Sira Carolin Günther, Martin Lothar Biniossek, Christoph Peters et al. The secreted inhibitor of invasive cell growth CREG1 is negatively regulated by cathepsin proteases Cellular and Molecular Life Sciences 1/1/2021 [PMID: 32385587]
• Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015-02-15;14(0):39. 2015-02-15 [PMID: 25744631] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Ctsz
• Uniprot:
  • Q9WUU7()