Cathepsin S Antibody - BSA Free

Western Blot: Cathepsin S Antibody [NBP2-15727] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Cathepsin S Antibody [NBP2-15727] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Cathepsin S antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP2-15727] - Cathepsin S antibody detects Cathepsin S protein at lysosome on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Cathepsin S antibody dilution: 1:500.

Western Blot: Cathepsin S Antibody [NBP2-15727] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody (NBP2-15727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cathepsin S Antibody [NBP2-15727] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody (NBP2-15727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cathepsin S Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cathepsin S Antibody - BSA Free (NBP2-15727) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cathepsin S. The exact sequence is proprietary.
• Localization: Lysosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTSS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (85%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cathepsin S Antibody - BSA Free

• Cathepsin S
• CTSS
• EC 3.4.22
• EC 3.4.22.27
• FLJ50259
• MGC3886

Background

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CTSS