Cathepsin L Antibody (PSH03-92)

Western Blot: Cathepsin L Antibody (PSH03-92) [NBP3-32112] - Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (NBP3-32112) at 1/5,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: HEK-293 cell lysate (15 ug/Lane) Lane 4: MDA-MB-231 cell lysate (15 ug/Lane) Lane 5: Human kidney tissue lysate (30 ug/Lane) Lane 6: Human liver tissue lysate (30 ug/Lane) Lane 7: NIH/3T3 cell lysate (15 ug/Lane) Lane 8: PC-12 cell lysate (15 ug/Lane) Lane 9: Mouse kidney tissue lysate (30 ug/Lane) Lane 10: Rat kidney tissue lysate (30 ug/Lane) Lane 11: Mouse liver tissue lysate (30 ug/Lane) Lane 12: Rat liver tissue lysate (30 ug/Lane) Lane 13: COS-1 cell lysate (15 ug/Lane) Predicted band size: 38 kDa Observed band size: 20-45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cathepsin L Antibody (PSH03-92) [NBP3-32112] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L antibody (NBP3-32112) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clone: PSH03-92
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cathepsin L Antibody (PSH03-92) Summary

• Description: Novus Biologicals Rabbit Cathepsin L Antibody (PSH03-92) (NBP3-32112) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human Cathepsin L aa 84-333 / 333. (Uniprot: P07711)
• Localization: Lysosome, Apical cell membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule, Secreted, extracellular space.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CTSL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10000
  • Western Blot 1:5000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cathepsin L Antibody (PSH03-92)

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTSL