Cathepsin L Antibodies: Novus Biologicals

AF1515

Cathepsin L Antibody [Unconjugated]

100 μg

AF1515

HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Cathepsin L was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse liver tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin L at approximately 51 and 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Cathepsin L was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Acute, first window RIC activates autophagy signaling via p-AMPK upregulation and concomitant downregulation of mTOR.(A) Western blots for autophagy related signaling proteins. (B) Quantification of the protein fold change in 1W RIC compared to 1W controls. Values are means ± S.E.M. n = 6–8 per group. An (*) denotes a statistically significant difference (P<0.05) compared to control. (P-: phospho-). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25347774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chronic, third window RIC increases the expression of autophagosome proteins, LC3I/II and Atg5.(A) Western blots for autophagy related signaling proteins. (B) Quantification of the protein fold change in 3W RIC compared to 3W controls. Values are means ± S.E.M. n = 6–8 per group. An (*) denotes a statistically significant difference (P<0.05) compared to control. (P-: phospho-). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25347774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of CTSV induces cellular senescence. (A) HUVECs were treated with si-negative control (NC), si-CTSV, si-ALDH1A2 or si-CTSV + si-ALDH1A2 for 24 h and simulated with 100 nM doxorubicin (DOX) for 24 h after incubated in complete growth medium for 24 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, si-CTSV and si-ALDH1A2 (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53 and P16 in HUVECs treated with DOX and siRNAs (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6 and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX and siRNAs (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ctsl expression is decreased in senescent aorta. (A) Representative images of hematoxylin-eosin staining in the thoracic aortas of aged (18-month-old) and young (8-week-old) mice (scale bar = 200 μm in top and 50 μm in bottom, respectively). (B) Representative images of Masson’s trichrome stain in the thoracic aortas (blue-gray staining for the collagens, scale bar = 200 μm in top and 50 μm in bottom, respectively). (C) Quantification of media thickness (MT), the ratio of media thickness to lumen diameter (MT/LD), media area (MA), and the ratio of collagen to media area (collagen/MA) using hematoxylin-eosin staining and Masson’s trichrome stain (n = 4). (D). Immunohistochemistry of Ctsl in the thoracic aortas of aged and young mice (brown staining for Ctsl, scale bar = 50 μm, n = 4). (E) Western Blot of Ctsl and p21 in young and senescent aortas (n = 3–4). Data were presented as mean ± SEM. T-test was used. * p < 0.05 vs. young, ** p < 0.01 vs. young, *** p < 0.001 vs. young. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

CTSV attenuates DOX-induced cellular senescence. (A). HUVECs were treated with ad-negative control (NC) or ad-CTSV (MOI = 10) for 8 h and simulated with 100 nM doxorubicin (DOX) and 1 μM RA for 24 h after incubated in complete growth medium for 40 h. SA-beta -gal activity was analyzed (blue staining for the senescent cells, scale bar = 50 μm, n = 4). (B) EdU assay of the cell proliferation ability in HUVECs treated with DOX, RA and ad-CTSV (red staining for the EdU, blue staining for Hoechst, scale bar = 25 μm, n = 4). (C) Representative image of the wound healing assay and the quantification of wound area in HUVECs (scale bar = 100 μm, n = 4). (D) Western blot of CTSV, ALDH1A2, P21, p-ERK1/2, ERK1/2, p-AKT, AKT, CTSL, P53, and P16 in HUVECs treated with DOX, RA and ad-CTSV (n = 4). (E) Relative mRNA level of CTSV, ALDH1A2, P21, IL-1 beta , IL-6, and ICAM-1 (n = 4). (F) RA concentration in HUVECs treated with DOX, RA, and ad-CTSV (n = 4). Data are presented as mean ± SEM. One-way ANOVA test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36671735), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1515U-100UG
Alexa Fluor 405	AF1515V-100UG
Alexa Fluor 488	AF1515G-100UG
Alexa Fluor 532	AF1515X-100UG
Alexa Fluor 594	AF1515T-100UG
Alexa Fluor 647	AF1515R-100UG
Alexa Fluor 700	AF1515N-100UG
Alexa Fluor 750	AF1515S-100UG
Alexa Fluor Plus 405	AF1515AFP405-100UG
Alexa Fluor Plus 488	AF1515AFP488-100UG
Alexa Fluor Plus 555	AF1515AFP555-100UG
Alexa Fluor Plus 594	AF1515AFP594-100UG
Alexa Fluor Plus 647	AF1515AFP647-100UG
Alexa Fluor Plus 680	AF1515AFP680-100UG
Allophycocyanin	AF1515A
Biotin	BAF1515
CoraFluor 1	AF1515CL1
DyLight 350	AF1515D
DyLight 405	AF1515E
DyLight 488	AF1515K
DyLight 550	AF1515L
DyLight 594	AF1515M
DyLight 650	AF1515W
DyLight 680	AF1515Y
DyLight 755	AF1515Z
FITC	AF1515F
HRP	AF1515H
Janelia Fluor 525	AF1515JF525
Janelia Fluor 549	AF1515I
Janelia Fluor 646	AF1515J
Janelia Fluor 669	AF1515JF669
PE	AF1515P
PerCP	AF1515C
mFluor Violet 450 SE	AF1515MFV450
mFluor Violet 500 SE	AF1515MFV500
mFluor Violet 610 SE	AF1515MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1515-SP

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AF952

Cathepsin L Antibody [Unconjugated]

(28 Publications)

100 μg

AF952

952-CY), Recombinant Mouse Cathepsin L (1515-CY), Recombinant Human Cathepsin V (1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (1183-CY), Recombinant Human Cathepsin H (7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 10 ng of Recombinant Human Cathepsin L (<a class=

952-CY), Recombinant Mouse Cathepsin L (1515-CY), Recombinant Human Cathepsin V (1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (1183-CY), Recombinant Human Cathepsin H (7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 10 ng of Recombinant Human Cathepsin L (952-CY), Recombinant Mouse Cathepsin L (1515-CY), Recombinant Human Cathepsin V (1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (1183-CY), Recombinant Human Cathepsin H (7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Cathepsins are reduced in GNPTAB- knockout cells, but restored upon GNPTAB reconstitution. a Immunoblotting of lysates from parental, knockout and reconstituted cells for CatB and CatL. The migration of a 30 kDa molecular mass marker is shown to the right. b CatB activity in lysates from parental HAP1, or knockout GNPTAB- or NPC1-cells. Cells were treated with cathepsin B inhibitor (Bi), cathepsin L inhibitor (Li), at 10 or 1 µM, or DMSO vehicle control for 1 h at 37 °C, before lysis and incubation with fluorescent CatB peptide substrate. After 1 h incubation at room temperature, fluorescence was measured. Data represent the mean ± s.d. of three technical replicates. A representative of three independent experiments is shown. c CatB activity in lysates from reconstituted cells. Lysates from transduced HAP1 or GNPTAB− cells were generated and tested for CatB activity, as for panel b. Data represent the mean ± s.d. of three technical replicates. A representative of two independent experiments is shown. For both panels b and c, statistical analysis was performed with a two-tailed Student’s t-test with significance shown as **** P ≤ 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30655525), licensed under a CC-BY license. Not internally tested by R&D Systems.

$<b>Biological Strategies Validation. </b>Legumain, cathepsin B and L expressions in cytosolic and membrane fractions of myotubes.Myotubes were treated with (+) or without (−) 30 µM simvastatin for 48 h before subcellular fractionation was performed. One representative immunoblot of legumain, cathepsin B and L in the cytosolic and membrane fractions is shown. All lanes were loaded with equal amount of total proteins and probed with antibodies as indicated. LAMP-2 and alpha -tubulin are shown as cell compartment controls (n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24416446), licensed under a CC-BY license. Not internally tested by R&D Systems.$

952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Cathepsin L (Catalog # <a class=

952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF952U-100UG
Alexa Fluor 405	AF952V-100UG
Alexa Fluor 488	AF952G-100UG
Alexa Fluor 532	AF952X-100UG
Alexa Fluor 594	AF952T-100UG
Alexa Fluor 647	AF952R-100UG
Alexa Fluor 700	AF952N-100UG
Alexa Fluor 750	AF952S-100UG
Alexa Fluor Plus 405	AF952AFP405-100UG
Alexa Fluor Plus 488	AF952AFP488-100UG
Alexa Fluor Plus 555	AF952AFP555-100UG
Alexa Fluor Plus 594	AF952AFP594-100UG
Alexa Fluor Plus 647	AF952AFP647-100UG
Alexa Fluor Plus 680	AF952AFP680-100UG
Allophycocyanin	AF952A
Biotin	BAF952
CoraFluor 1	AF952CL1
DyLight 350	AF952D
DyLight 405	AF952E
DyLight 488	AF952K
DyLight 550	AF952L
DyLight 594	AF952M
DyLight 650	AF952W
DyLight 680	AF952Y
DyLight 755	AF952Z
FITC	AF952F
HRP	AF952H
Janelia Fluor 525	AF952JF525
Janelia Fluor 549	AF952I
Janelia Fluor 646	AF952J
Janelia Fluor 669	AF952JF669
PE	AF952P
PerCP	AF952C
mFluor Violet 450 SE	AF952MFV450
mFluor Violet 500 SE	AF952MFV500
mFluor Violet 610 SE	AF952MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF952-SP

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MAB9521

Cathepsin L Antibody (204101) [Unconjugated]

(13 Publications)

100 μg

MAB9521

952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." class="big_lightbox">

Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # <a class=

952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." alt="Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9521U-100UG
Alexa Fluor 405	FAB9521V-100UG
Alexa Fluor 488	FAB9521G-100UG
Alexa Fluor 532	FAB9521X-100UG
Alexa Fluor 594	FAB9521T-100UG
Alexa Fluor 647	FAB9521R-100UG
Alexa Fluor 700	FAB9521N-100UG
Alexa Fluor 750	FAB9521S-100UG
Alexa Fluor Plus 405	FAB9521AFP405-100UG
Alexa Fluor Plus 488	FAB9521AFP488-100UG
Alexa Fluor Plus 555	FAB9521AFP555-100UG
Alexa Fluor Plus 594	FAB9521AFP594-100UG
Alexa Fluor Plus 647	FAB9521AFP647-100UG
Alexa Fluor Plus 680	FAB9521AFP680-100UG
Allophycocyanin	FAB9521A
Biotin	FAB9521B
CoraFluor 1	FAB9521CL1
DyLight 350	FAB9521D
DyLight 405	FAB9521E
DyLight 488	FAB9521K
DyLight 550	FAB9521L
DyLight 594	FAB9521M
DyLight 650	FAB9521W
DyLight 680	FAB9521Y
DyLight 755	FAB9521Z
FITC	FAB9521F
HRP	FAB9521H
Janelia Fluor 525	FAB9521JF525
Janelia Fluor 549	FAB9521I
Janelia Fluor 646	FAB9521J
Janelia Fluor 669	FAB9521JF669
PE	FAB9521P
PerCP	FAB9521C
mFluor Violet 450 SE	FAB9521MFV450
mFluor Violet 500 SE	FAB9521MFV500
mFluor Violet 610 SE	FAB9521MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9521-SP
Carrier Free	MAB9521-500

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MAB22591

Cathepsin L Antibody (193702)

(17 Publications)

100 μg

Reactivity:

I

Species Glossary

Reactivity: I

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB22591U-100UG
Alexa Fluor 405	FAB22591V-100UG
Alexa Fluor 488	FAB22591G-100UG
Alexa Fluor 532	FAB22591X-100UG
Alexa Fluor 594	FAB22591T-100UG
Alexa Fluor 647	FAB22591R-100UG
Alexa Fluor 700	FAB22591N-100UG
Alexa Fluor 750	FAB22591S-100UG
Alexa Fluor Plus 405	FAB22591AFP405-100UG
Alexa Fluor Plus 488	FAB22591AFP488-100UG
Alexa Fluor Plus 555	FAB22591AFP555-100UG
Alexa Fluor Plus 594	FAB22591AFP594-100UG
Alexa Fluor Plus 647	FAB22591AFP647-100UG
Alexa Fluor Plus 680	FAB22591AFP680-100UG
Allophycocyanin	FAB22591A
Biotin	FAB22591B
CoraFluor 1	FAB22591CL1
DyLight 350	FAB22591D
DyLight 405	FAB22591E
DyLight 488	FAB22591K
DyLight 550	FAB22591L
DyLight 594	FAB22591M
DyLight 650	FAB22591W
DyLight 680	FAB22591Y
DyLight 755	FAB22591Z
FITC	FAB22591F
HRP	FAB22591H
Janelia Fluor 525	FAB22591JF525
Janelia Fluor 549	FAB22591I
Janelia Fluor 646	FAB22591J
Janelia Fluor 669	FAB22591JF669
PE	FAB22591P
PerCP	FAB22591C
mFluor Violet 450 SE	FAB22591MFV450
mFluor Violet 500 SE	FAB22591MFV500
mFluor Violet 610 SE	FAB22591MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB22591-SP

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NB100-1775

Cathepsin L Antibody (33/2)

(7 Publications)

0.1 ml

NB100-1775

Reactivity:

Hu, Mu, Rt, Md

Species Glossary

Reactivity: Hu, Mu, Rt, Md

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB952

Cathepsin L Antibody (204106) [Unconjugated]

(2 Publications)

500 μg

MAB952

952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human Cathepsin L Monoclonal Antibody (Catalog # MAB952) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human Cathepsin L Monoclonal Antibody (Catalog # MAB952) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human Cathepsin L Monoclonal Antibody (Catalog # MAB952) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB952U-100UG
Alexa Fluor 405	FAB952V-100UG
Alexa Fluor 488	FAB952G-100UG
Alexa Fluor 532	FAB952X-100UG
Alexa Fluor 594	FAB952T-100UG
Alexa Fluor 647	FAB952R-100UG
Alexa Fluor 700	FAB952N-100UG
Alexa Fluor 750	FAB952S-100UG
Alexa Fluor Plus 405	FAB952AFP405-100UG
Alexa Fluor Plus 488	FAB952AFP488-100UG
Alexa Fluor Plus 555	FAB952AFP555-100UG
Alexa Fluor Plus 594	FAB952AFP594-100UG
Alexa Fluor Plus 647	FAB952AFP647-100UG
Alexa Fluor Plus 680	FAB952AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB952-SP

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NBP3-32112

Cathepsin L Antibody (PSH03-92)

100 ul

NBP3-32112

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB2259

Cathepsin L Antibody (193720)

(2 Publications)

500 μg

Reactivity:

I

Species Glossary

Reactivity: I

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2259U-100UG
Alexa Fluor 405	FAB2259V-100UG
Alexa Fluor 488	FAB2259G-100UG
Alexa Fluor 532	FAB2259X-100UG
Alexa Fluor 594	FAB2259T-100UG
Alexa Fluor 647	FAB2259R-100UG
Alexa Fluor 700	FAB2259N-100UG
Alexa Fluor 750	FAB2259S-100UG
Alexa Fluor Plus 405	FAB2259AFP405-100UG
Alexa Fluor Plus 488	FAB2259AFP488-100UG
Alexa Fluor Plus 555	FAB2259AFP555-100UG
Alexa Fluor Plus 594	FAB2259AFP594-100UG
Alexa Fluor Plus 647	FAB2259AFP647-100UG
Alexa Fluor Plus 680	FAB2259AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2259-SP

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NBP3-21333

Cathepsin L Antibody - BSA Free

100 ul

NBP3-21333

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil

Staining of human cell line A549 shows localization to the Golgi apparatus & vesicles.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21333-25ul

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NBP2-89528

Cathepsin L Antibody (221) - Azide and BSA Free

100 ul

NBP2-89528

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89528AF350
Alexa Fluor 405	NBP2-89528AF405
Alexa Fluor 488	NBP2-89528AF488
Alexa Fluor 532	NBP2-89528AF532
Alexa Fluor 594	NBP2-89528AF594
Alexa Fluor 647	NBP2-89528AF647
Alexa Fluor 700	NBP2-89528AF700
Alexa Fluor 750	NBP2-89528AF750
Alexa Fluor Plus 405	NBP2-89528AFP405
Alexa Fluor Plus 488	NBP2-89528AFP488
Alexa Fluor Plus 555	NBP2-89528AFP555
Alexa Fluor Plus 594	NBP2-89528AFP594
Alexa Fluor Plus 647	NBP2-89528AFP647
Alexa Fluor Plus 680	NBP2-89528AFP680
Allophycocyanin	NBP2-89528APC
Biotin	NBP2-89528B
CoraFluor 1	NBP2-89528CL1
DyLight 350	NBP2-89528UV
DyLight 405	NBP2-89528V
DyLight 488	NBP2-89528G
DyLight 550	NBP2-89528R
DyLight 594	NBP2-89528DL594
DyLight 650	NBP2-89528C
DyLight 680	NBP2-89528FR
DyLight 755	NBP2-89528IR
FITC	NBP2-89528F
HRP	NBP2-89528H
Janelia Fluor 525	NBP2-89528JF525
Janelia Fluor 549	NBP2-89528JF549
Janelia Fluor 646	NBP2-89528JF646
Janelia Fluor 669	NBP2-89528JF669
PE	NBP2-89528PE
PerCP	NBP2-89528PCP
mFluor Violet 450 SE	NBP2-89528MFV450
mFluor Violet 500 SE	NBP2-89528MFV500
mFluor Violet 610 SE	NBP2-89528MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89528-50ul

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NBP3-43274

Cathepsin L Antibody - BSA Free

100 ul

Reactivity:

Bv

Species Glossary

Reactivity: Bv

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-89527

Cathepsin L Antibody (002) - Azide and BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89527AF350
Alexa Fluor 405	NBP2-89527AF405
Alexa Fluor 488	NBP2-89527AF488
Alexa Fluor 532	NBP2-89527AF532
Alexa Fluor 594	NBP2-89527AF594
Alexa Fluor 647	NBP2-89527AF647
Alexa Fluor 700	NBP2-89527AF700
Alexa Fluor 750	NBP2-89527AF750
Alexa Fluor Plus 405	NBP2-89527AFP405
Alexa Fluor Plus 488	NBP2-89527AFP488
Alexa Fluor Plus 555	NBP2-89527AFP555
Alexa Fluor Plus 594	NBP2-89527AFP594
Alexa Fluor Plus 647	NBP2-89527AFP647
Alexa Fluor Plus 680	NBP2-89527AFP680
Allophycocyanin	NBP2-89527APC
Biotin	NBP2-89527B
CoraFluor 1	NBP2-89527CL1
DyLight 350	NBP2-89527UV
DyLight 405	NBP2-89527V
DyLight 488	NBP2-89527G
DyLight 550	NBP2-89527R
DyLight 594	NBP2-89527DL594
DyLight 650	NBP2-89527C
DyLight 680	NBP2-89527FR
DyLight 755	NBP2-89527IR
FITC	NBP2-89527F
HRP	NBP2-89527H
Janelia Fluor 525	NBP2-89527JF525
Janelia Fluor 549	NBP2-89527JF549
Janelia Fluor 646	NBP2-89527JF646
Janelia Fluor 669	NBP2-89527JF669
PE	NBP2-89527PE
PerCP	NBP2-89527PCP
mFluor Violet 450 SE	NBP2-89527MFV450
mFluor Violet 500 SE	NBP2-89527MFV500
mFluor Violet 610 SE	NBP2-89527MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89527-50ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Cathepsin L Antibodies

Cathepsin L Antibody [Unconjugated]

Cathepsin L Antibody [Unconjugated]

Cathepsin L Antibody (204101) [Unconjugated]

Cathepsin L Antibody (193702)

Cathepsin L Antibody (33/2)

Cathepsin L Antibody (204106) [Unconjugated]

Cathepsin L Antibody (PSH03-92)

Cathepsin L Antibody (193720)

Cathepsin L Antibody - BSA Free

Cathepsin L Antibody (221) - Azide and BSA Free

Cathepsin L Antibody - BSA Free

Cathepsin L Antibody (002) - Azide and BSA Free