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HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
952-CY), Recombinant Mouse Cathepsin L (1515-CY), Recombinant Human Cathepsin V (1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (1183-CY), Recombinant Human Cathepsin H (7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." class="big_lightbox" target="_blank">
CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF1515_Western_Blot_19034.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF952_Western_Blot_20998.jpg)
![Western Blot Cathepsin L Antibody (204101) [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_MAB9521_Western_Blot_20999.jpg)
![N/A Cathepsin L [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Cathepsin_L_DY952_ELISA_2273.jpg)
![Immunohistochemistry Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF1515_Immunohistochemistry_6704.jpg)
![Simple Western Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF1515_Simple_Western_18967.jpg)
![Immunohistochemistry Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF1515_Immunohistochemistry_7059.jpg)
![Immunocytochemistry/ Immunofluorescence Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-8220249505999.jpg)
![Immunocytochemistry/ Immunofluorescence Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-822024102441.jpg)
![Immunocytochemistry/ Immunofluorescence Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-44202415425088.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-121220257481322.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-121220255495813.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-121220259528.jpg)
![Immunohistochemistry Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-immunohistochemistry-12122025974213.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-121220258545215.jpg)
![Immunohistochemistry Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-immunohistochemistry-12122025857414.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-1212202591403.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1515_mouse-rat-cathepsin-l-affinity-purified-polyclonal-ab-western-blot-1212202592080.jpg)
![Immunohistochemistry Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_AF952_Immunohistochemistry_17794.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af952_human-cathepsin-l-affinity-purified-polyclonal-ab-8220249511428.jpg)
![Western Blot Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af952_human-cathepsin-l-affinity-purified-polyclonal-ab-8220249504626.jpg)
![ELISA Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/af952_human-cathepsin-l-affinity-purified-polyclonal-ab-elisa-639201781558723860.jpg)
![ELISA Cathepsin L Antibody [Unconjugated]](http://images.novusbio.com/images/af952_human-cathepsin-l-affinity-purified-polyclonal-ab-elisa-639201850085787215.jpg)
![Immunohistochemistry Cathepsin L Antibody (204101) [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_MAB9521_Immunohistochemistry_16928.jpg)
![Immunohistochemistry Cathepsin L Antibody (204101) [Unconjugated]](http://images.novusbio.com/images/antibody/Cathepsin_L_MAB9521_Immunohistochemistry_16929.jpg)
![Enzyme Activity Cathepsin L [Unconjugated]](http://images.novusbio.com/images/952-cy_recombinant-human-cathepsin-l-protein-cf-enzyme-activity-962026105156.jpg)
