Cathepsin L Antibody (221) - Azide and BSA Free

Western Blot: Cathepsin L Antibody (221) [NBP2-89528] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLane C: HCT116 Whole Cell Lysate Lane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDaObserved band size:25 kDa

NBP2-89528 used at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lane B: Cathepsin L knockout A549 Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 25 kDa.

• Reactivity: Hu
• Applications: WB
• Clone: 221
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cathepsin L Antibody (221) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Cathepsin L (Accession#: NP_001903.1; Met1-Val333).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CTSL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Cathepsin L Antibody (221) - Azide and BSA Free

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTSL