Cathepsin F Antibody

Western Blot: Cathepsin F Antibody [NB100-1784] - (0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cathepsin F Antibody Summary

• Description: Novus Biologicals Goat Cathepsin F Antibody (NB100-1784) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cathepsin F Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CGVNTMASSAVVD corresponding to C-Terminus according to NP_003784.2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTSF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 30 kDa band and 35 kDa band in human prostate and ovary lysates after 0.3ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 9822672). This protein has a calculated MW of 53.4 kDa band according to NP_003784.2.
• Control:

  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  Human Prostate Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cathepsin F Antibody

• Cathepsin F
• CATSFEC 3.4.22.41
• CTSF
• EC 3.4.22

Background

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin F Antibody (NB100-1784)(3)

Publications using NB100-1784

• Maubach G, Lim MCC, Zhuo L. Nuclear Cathepsin F Regulates Activation Markers in Rat Hepatic Stellate Cells. Mol Biol Cell :E08-03-0291. 2008-01-01 [PMID: 18667530]
• da Silva Ferreira ME, Savoldi M, Sueli Bonato P et al. Fungal metabolic model for tyrosinemia type 3: molecular characterization of a gene encoding a 4-hydroxy-phenyl pyruvate dioxygenase from Aspergillus nidulans. Eukaryot Cell 2006-08-01 [PMID: 16896227]
• Dorn A, Zhao H, Granberg F et al. Identification of specific cellular genes up-regulated late in adenovirus type 12 infection. J Virol 2005-02-01 [PMID: 15681441]

Bioinformatics

• Gene Symbol: CTSF
• Entrez:
  • 8722(Human)
• Uniprot:
  • NP_003784.2(Human)