Human Pro-MMP-10 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Pro-MMP-10 Quantikine ELISA Kit Summary

• Background: The Quantikine Human Pro-MMP-10 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure pro-MMP-10 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human pro-MMP-10 and antibodies raised against the recombinant protein. This kit will not detect human active MMP-10 existing in either free or TIMP-bound form. Natural human pro-MMP-10 showed dos...e-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-10.
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• Specificity: Natural and recombinant human pro-MMP-10
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MMP10

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pro-MMP-10 Quantikine ELISA Kit

• EC 3.4.24
• EC 3.4.24.22
• matrix metallopeptidase 10 (stromelysin 2)
• matrix metalloprotease 10
• matrix metalloproteinase 10 (stromelysin 2)
• Matrix metalloproteinase-10
• MMP10
• MMP-10
• SL-2
• STMY2stromelysin-2
• Stromelysin 2
• transin 2
• transin-2

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-10 (DM1000)(12)

Publications using DM1000

• JM Mora-Gutié, JA Rodríguez, MA Fernández-, J Orbe, FJ Escalada, MJ Soler, MF Slon Roble, M Riera, JA Páramo, N Garcia-Fer MMP-10 is Increased in Early Stage Diabetic Kidney Disease and can be Reduced by Renin-Angiotensin System Blockade Sci Rep, 2020;10(1):26. 2020 [PMID: 31913319] (Human)
• Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med, 2016;14(1):31. 2016 [PMID: 26830497] (Human)
• Chen , Chang-Ha, Chang , Alice Y, Li , Shau-Hsu, Tsai , Hsin-Tin, Shiu , Li-Yen, Su , Li-Jen, Wang , Wen-Lung, Chiu , Tai-Jen, Luo , Sheng-De, Huang , Tai-Lin, Chien , Chih-Yen Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer. Mol Cancer, 2015;14(0):83. 2015 [PMID: 25889801] (Human)
• Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer. Clin Cancer Res, 2014;20(24):6324-35. 2014 [PMID: 25326231] (Human)
• Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2010;84(3):239-51. 2010 [PMID: 19922462] (Human)
• Lorente L, Martin MM, Labarta L, Diaz C, Sole-Violan J, Blanquer J, Orbe J, Rodriguez JA, Jimenez A, Borreguero-Leon JM, Belmonte F, Medina JC, Lliminana MC, Ferrer-Aguero JM, Ferreres J, Mora ML, Lubillo S, Sanchez M, Barrios Y, Sierra A, Paramo JA Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis. Crit Care, 2009;13(5):R158. 2009 [PMID: 19799791] (Human)
• McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy? Kidney Int., 2007;72(4):481-8. 2007 [PMID: 17554258] (Human)
• Barksby HE, Milner JM, Patterson AM, Peake NJ, Hui W, Robson T, Lakey R, Middleton J, Cawston TE, Richards CD, Rowan AD Matrix metalloproteinase 10 promotion of collagenolysis via procollagenase activation: implications for cartilage degradation in arthritis. Arthritis Rheum., 2006;54(10):3244-53. 2006 [PMID: 17009259] (Human)
• Montero I, Orbe J, Varo N, Beloqui O, Monreal JI, Rodriguez JA, Diez J, Libby P, Paramo JA C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis. J. Am. Coll. Cardiol., 2006;47(7):1369-78. 2006 [PMID: 16580524] (Human)
• Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis. Infect. Immun., 2005;73(1):126-34. 2005 [PMID: 15618147] (Human)
• Singh S, Singh UP, Grizzle WE, Lillard JW CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion. Lab. Invest., 2004;84(12):1666-76. 2004 [PMID: 15467730] (Human)
• Ramos MC, Steinbrenner H, Stuhlmann D, Sies H, Brenneisen P Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation. Biol. Chem., 2004;385(1):75-86. 2004 [PMID: 14977049] (Human)

Bioinformatics

• Gene Symbol: MMP10