Cathepsin X/Z/P Antibody [Unconjugated]

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin X/Z/P at approximately 36 kDa (as indicated) using 5 µg/mL ...read more
Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of normal human breast and breast cancer tissue using Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Cathepsin X/Z/P Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Specificity
Detects human Cathepsin X/Z/P in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Cathepsin C is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSZ
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF934 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin X/Z/P Antibody [Unconjugated]

  • Cathepsin P
  • Cathepsin X/Z/P
  • Cathepsin Z
  • CTSZ

Background

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

  1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
  2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
  3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
  4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
  5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
  6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, R. et al. (2001) Biol. Chem. 382:839.
  8. Therrien, C. et al. (2001) Biochemistry 40:2702.
  9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin X/Z/P Antibody (AF934)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, Flow Cytometry, ICC, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(2)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF934 Applications Species
Y Aiba, K Harada, M Ito, T Suematsu, S Aishima, Y Hitomi, N Nishida, M Kawashima, M Takatsuki, S Eguchi, S Shimoda, H Nakamura, A Komori, S Abiru, S Nagaoka, K Migita, H Yatsuhashi, K Tokunaga, M Nakamura Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis Sci Rep, 2018;8(1):11808. 2018 [PMID: 30087368] (ICC, Human) ICC Human
P Anja, J Anahid, K Janko Cysteine cathepsins: Their biological and molecular significance in cancer stem cells Semin. Cancer Biol., 2018;0(0):. 2018 [PMID: 30063965] (ICC, Human) ICC Human
Pecar Fonovic U, Kos J Cathepsin X Cleaves Profilin 1 C-Terminal Tyr139 and Influences Clathrin-Mediated Endocytosis. PLoS ONE, 2015;10(9):e0137217. 2015 [PMID: 26325675] (Western Blot, Human) Western Blot Human
Akkari L, Gocheva V, Kester J, Hunter K, Quick M, Sevenich L, Wang H, Peters C, Tang L, Klimstra D, Reinheckel T, Joyce J Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix. Genes Dev, 2014;28(19):2134-50. 2014 [PMID: 25274726] (IHC-P, Human) IHC-P Human
Vizin T, Christensen I, Wilhelmsen M, Nielsen H, Kos J Prognostic and predictive value of cathepsin X in serum from colorectal cancer patients. BMC Cancer, 2014;14(0):259. 2014 [PMID: 24725597] (ELISA Development, Human) ELISA Development Human
Jevnikar Z, Mirkovic B, Fonovic U, Zidar N, Svajger U, Kos J Three-dimensional invasion of macrophages is mediated by cysteine cathepsins in protrusive podosomes. Eur J Immunol, 2012;42(12):3429-41. 2012 [PMID: 23018451] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

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Biotin BAF934

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Bioinformatics

Gene Symbol CTSZ
Uniprot