Cathepsin E Antibody

Cathepsin E was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse Cathepsin E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1130) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of type II alveolar cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin E Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
  Gln19-Pro397
  Accession # P70269
• Specificity: Detects both pro and mature mouse Cathepsin E in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human Cathepsin E and less than 1% cross-reactivity with recombinant mouse Cathepsin D is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTSE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin E Antibody

• CATE
• Cathepsin E
• CTSE
• EC 3.4.23
• EC 3.4.23.34
• erythrocyte membrane aspartic proteinase
• slow-moving proteinase

Background

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin E Antibody (AF1130)(5)

Publications using AF1130

• Malgorzata Ciurkiewicz, Stefan Floess, Michael Beckstette, Maren Kummerfeld, Wolfgang Baumgärtner, Jochen Huehn et al. Transcriptome analysis following neurotropic virus infection reveals faulty innate immunity and delayed antigen presentation in mice susceptible to virus‐induced demyelination Brain Pathology 11/1/2021 [PMID: 34231271]
• Harada Y, Zhang J, Imari K et al. Cathepsin E in neutrophils contributes to the generation of neuropathic pain in experimental autoimmune encephalomyelitis Pain 9/1/2019 [PMID: 31095099]
• SL Ivry, JM Sharib, DA Dominguez, N Roy, SE Hatcher, M Yip-Schnei, CM Schmidt, RE Brand, WG Park, M Hebrok, G Kim, AJ O'Donoghue, KS Kirkwood, CS Craik Global protease activity profiling provides differential diagnosis of pancreatic cysts Clin. Cancer Res., 2017-04-19;0(0):. 2017-04-19 [PMID: 28424202] (IHC-P, Mouse)
• Anthony J. O’Donoghue, Sam L. Ivry, Chaity Chaudhury, Daniel R. Hostetter, Douglas Hanahan, Charles S. Craik Procathepsin E is highly abundant but minimally active in pancreatic ductal adenocarcinoma tumors Biological Chemistry 9/1/2016 [PMID: 27149201]
• Buhling F, Kouadio M, Chwieralski CE, Kern U, Hohlfeld JM, Klemm N, Friedrichs N, Roth W, Deussing JM, Peters C, Reinheckel T Gene targeting of the cysteine peptidase cathepsin H impairs lung surfactant in mice. PLoS ONE, 2011-10-12;6(10):e26247. 2011-10-12 [PMID: 22022579] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CTSE
• Uniprot:
  • P70269()