Cathepsin E Antibody (212211) [Alexa Fluor® 750]

• Applications: WB, IP
• Clone: 212211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Cathepsin E Antibody (212211) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin E
  Gln18-Pro396
  Accession # NP_001901
• Specificity: Detects human Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and mature forms of recombinant human (rh) Cathepsin E. In Western blots, 100% cross‑reactivity with recombinant mouse (rm) Cathepsin&nb
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta  site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (aa 1‑17), a propeptide (aa 18‑53), and a mature chain (aa 54‑396) (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.