Cathepsin D Antibody [Unconjugated]

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) ...read more
Western blot shows lysates of PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Procathepsin D at ...read more
Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Cathepsin D Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin D
Leu21-Leu412
Accession # P07339
Specificity
Detects human Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) Cathepsin D is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSD
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF1014 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody [Unconjugated]

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC 3.4.23.5
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10

Background

Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19-64), and a mature chain (residues 65‑412) (2‑4). The mature chain can be processed further to the light (residues 65‑161) and heavy (residues 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

  1. Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
  2. Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
  3. Westley and May (1987) Nucl. Acid Res. 15:3773.
  4. Redecker, et al. (1991) DNA Cell Biol. 10:423.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (AF1014)(7)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 4 applications: Electron Microscopy, IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
Electron Microscopy
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(6)
Canine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1014 Applications Species
C Bonolo De, YX Zhu, N Sepetov, S Romanov, LA Bruins, CX Shi, CK Stein, JL Petit, AN Polito, ME Sharik, EW Meermeier, GJ Ahmann, ID Lopez Arme, J Kruse, PL Bergsagel, M Chesi, N Meurice, E Braggio, AK Stewart Identification of PIKfyve kinase as a target in multiple myeloma Haematologica, 2019;0(0):. 2019 [PMID: 31582538] (Western Blot, Human) Western Blot Human
X Gao, A De Mazière, DB Iaea, CP Arthur, J Klumperman, C Ciferri, RN Hannoush Visualizing the cellular route of entry of a cystine-knot peptide with Xfect transfection reagent by electron microscopy Sci Rep, 2019;9(1):6907. 2019 [PMID: 31061420] (Electron Microscopy, Human) Electron Microscopy Human
J Han, LA Goldstein, W Hou, S Chatterjee, TF Burns, H Rabinowich HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC Autophagy, 2018;0(0):1-14. 2018 [PMID: 29561705] (Western Blot, Human) Western Blot Human
Amphiphilic star PEG-Camptothecin conjugates for intracellular targeting J Control Release, 2016;0(0):. 2016 [PMID: 27677603] (IHC-Fr, Human) IHC-Fr Human
The Apaf-1-binding protein Aven is cleaved by Cathepsin D to unleash its anti-apoptotic potential. Cell Death Differ., 2012;19(9):1435-45. 2012 [PMID: 22388353] (Western Blot, Human) Western Blot Human
Khalkhali-Ellis Z, Abbott DE, Bailey CM, Goossens W, Margaryan NV, Gluck SL, Reuveni M, Hendrix MJ IFN-gamma regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells. J. Cell. Biochem., 2008;105(1):208-18. 2008 [PMID: 18494001] (Western Blot, Human) Western Blot Human
Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E Clathrin is a key regulator of basolateral polarity. Nature, 2008;452(7188):719-23. 2008 [PMID: 18401403] (Immunoprecipitation, Canine) Immunoprecipitation Canine

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF1014

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Bioinformatics

Gene Symbol CTSD
Uniprot