Cathepsin D Antibody (204712) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB
• Clone: 204712
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

Cathepsin D Antibody (204712) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
  Ile21-Leu410
  Accession # P18242
• Specificity: Detects both the pro and active forms of mouse Cathepsin D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Cathepsin D or recombinant mouse Cathepsin E is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody (204712) [Alexa Fluor® 700]

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑64), and a mature chain (residues 65‑410) (1‑3). It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.