Cathepsin D Antibody (204712) [Alexa Fluor™ Plus 647]

• Reactivity: Mu
• Clone: 204712
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 647

Cathepsin D Antibody (204712) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects both the pro and active forms of mouse Cathepsin D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Cathepsin D or recombinant mouse Cathepsin E is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody (204712) [Alexa Fluor™ Plus 647]

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑64), and a mature chain (residues 65‑410) (1‑3). It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
4. Conner (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
6. Tsukuba, et al. (2000) Mol. Cells 10:601.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.