Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric)

ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Samples were spiked with high concentrations of Human Cathepsin A/Lysosomal Carboxypeptidase A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) [NBP3-00371] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.92%
• Intra-Assay: 5.61%
• Spike Recovery: 87 - 106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CTSA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cathepsin A/Lysosomal Carboxypeptidase A ELISA Kit (Colorimetric)

• beta-galactosidase 2
• beta-galactosidase protective protein
• Carboxypeptidase C
• Carboxypeptidase L
• Cathepsin A
• cathepsin ANGBE
• CTSA
• EC 3.4.16
• EC 3.4.16.5
• GLB2
• GSL
• Lysosomal Carboxypeptidase A
• lysosomal protective protein
• PPCA
• PPGBprotective protein for beta-galactosidase (galactosialidosis)
• Protective protein cathepsin A
• Protective protein for beta-galactosidase

Background

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CTSA