Cathepsin A/Lysosomal Carboxypeptidase A Antibody (RM0160-4A12) - Azide and BSA Free

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0160-4A12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Cathepsin A/Lysosomal Carboxypeptidase A Antibody (RM0160-4A12) - Azide and BSA Free Summary

• Immunogen: Mouse recombinant Cathepsin A/Lysosomal Carboxypeptidase A
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:400
  • Western Blot 1:500-1:1000
• Application Notes: This antibody may also work in ELISA. In western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (RM0160-4A12) - Azide and BSA Free

• beta-galactosidase 2
• beta-galactosidase protective protein
• Carboxypeptidase C
• Carboxypeptidase L
• Cathepsin A
• cathepsin ANGBE
• CTSA
• EC 3.4.16
• EC 3.4.16.5
• GLB2
• GSL
• Lysosomal Carboxypeptidase A
• lysosomal protective protein
• PPCA
• PPGBprotective protein for beta-galactosidase (galactosialidosis)
• Protective protein cathepsin A
• Protective protein for beta-galactosidase

Background

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cathepsin A/Lysosomal Carboxypeptidase A Antibody (NBP2-12071). (Showing 1 - 1 of 1 FAQ).

1. How should I reconstitute this antibody?
   • You should reconstitute this antibody in 200 uL sterile PBS.

Bioinformatics

• Gene Symbol: CTSA
• Uniprot:
  • P16675()