Catalase Antibody - BSA Free

Immunoprecipitation:[NBP3-29065]-Detection of human Catalase/CAT by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Catalase/CAT antibody used for IP at 6 µg per reaction. Catalase/CAT was also immunoprecipitated by rabbit anti-Catalase/CAT antibody. For blotting immunoprecipitated Catalase/CAT, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Catalase Antibody - BSA Free Summary

• Immunogen: Between 477 and 527
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Catalase Antibody - BSA Free

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAT