Caspase-7 Antibody (Mch3 1-1-11) - BSA Free

Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.

Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.

Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, KD
• Clone: Mch3 1-1-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Caspase-7 Antibody (Mch3 1-1-11) - BSA Free Summary

• Immunogen: Full-length recombinant human Caspase 7 [UniProt# P55210]
• Localization: Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Simple Western 1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at approx. 35 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat +Etoposide, HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, 1:500, apparent MW was 41, 44 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate
  Caspase-7 Overexpression Lysate
  Jurkat Staurosporine Treated / Untreated Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-7 Antibody (Mch3 1-1-11) - BSA Free

• apoptosis-related cysteine protease
• Apoptotic protease Mch-3
• CASP7
• CASP-7
• caspase 7, apoptosis-related cysteine peptidase
• Caspase7
• Caspase-7
• EC 3.4.22
• EC 3.4.22.60
• ICE-like apoptotic protease 3
• Lice2 alpha/beta/gamma
• Mch3

Background

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-7 Antibody (NB500-206)(2)

Publications using NB500-206

• Badr DM, Hafez HF, Agha AM, Shouman SA. The Combination of alpha-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit. Oxid Med Cell Longev. 2016-04-29 [PMID: 27127548] (WB, Human)
• Blanc-Brude OP et al. Therapeutic targeting of the survivin pathway in cancer: initiation of mitochondrial apoptosis and suppression of tumor-associated angiogenesis. Clin Cancer Res;9(7):2683-92. 2003-07-01 [PMID: 12855648]

Bioinformatics

• Gene Symbol: CASP7
• Entrez:
  • 840(Human)
  • 12369(Mouse)
  • 64026(Rat)
• Uniprot:
  • P55210(Mouse)