Caspase-7 Antibody (Mch3 1-1-11) - BSA Free

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Genetic Strategies: Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF ...read more
Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.
Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, KD
Clone
Mch3 1-1-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

Caspase-7 Antibody (Mch3 1-1-11) - BSA Free Summary

Immunogen
Full-length recombinant human Caspase 7 [UniProt# P55210]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Knockdown Validated
  • Simple Western 1:500
  • Western Blot 1:500-1:1000
Application Notes
This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at approx. 35 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat +Etoposide, HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, 1:500, apparent MW was 41, 44 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
HeLa Whole Cell Lysate
Caspase-7 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Publications
Read Publications using
NB500-206 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-7 Antibody (Mch3 1-1-11) - BSA Free

  • apoptosis-related cysteine protease
  • Apoptotic protease Mch-3
  • CASP7
  • CASP-7
  • caspase 7, apoptosis-related cysteine peptidase
  • Caspase7
  • Caspase-7
  • EC 3.4.22
  • EC 3.4.22.60
  • ICE-like apoptotic protease 3
  • Lice2 alpha/beta/gamma
  • Mch3

Background

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-7 Antibody (NB500-206)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for Caspase-7 Antibody (NB500-206): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Caspase-7 Products

Research Areas for Caspase-7 Antibody (NB500-206)

Find related products by research area.

Blogs on Caspase-7.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP7
Entrez
Uniprot