CART/CARTPT Antibody (113612) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 113612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

CART/CARTPT Antibody (113612) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human CART
  Ala37-Leu116
  Accession # Q16568
• Specificity: Detects human CART aa 28‑116 in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CART/CARTPT Antibody (113612) [Alexa Fluor® 700]

• CART prepropeptide
• CART
• CARTcocaine and amphetamine regulated transcript
• CARTPT
• cocaine- and amphetamine-regulated transcript protein

Background

CART, also known as cocaine and amphetamine-related transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations.  In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment.  There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region.  There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist.  These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89).  Human CART is 98% aa identical to both moyuse and rat CART (1-89).  Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions.   In addition, anatomical location is associated with differing lengths of CART.  For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.