Carm1 Antibody

Images

 
Genetic Strategies: Knockout Validated: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was ...read more
Western Blot: Carm1 Antibody [NB100-56374] - Analysis of PRMT4 in cell lysates from HeLa cells using this antibody. 5 ug/ml. Twenty ug of HeLa cell lysate was loaded per well of mini gel.
Immunocytochemistry/ Immunofluorescence: Carm1 Antibody [NB100-56374] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374] - Analysis of a FFPE tissue section of the human breast carcinoma using 1:200 dilution of Carm1 antibody (NB100-56374). The signal was developed using HRP-DAB ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Carm1 Antibody Summary

Immunogen
This antibody was developed against a mixture of synthetic peptides containing amino acids 45-69 and 595-608 of human PRMT4.
Predicted Species
Mouse (100%), Rat (100%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 0.5-2 ug/ml
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-56374.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Carm1 Antibody

  • Carm1
  • coactivator-associated arginine methyltransferase 1EC 2.1.1.-
  • EC 2.1.1
  • histone-arginine methyltransferase CARM1
  • PRMT4
  • PRMT4EC 2.1.1.125
  • Protein arginine N-methyltransferase 4

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. CARM1 (coactivator-associated arginine methyltransferase-1) also known as PRMT4 (protein-arginine N-methyltransferase-4) belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carm1 Antibody (NB100-56374)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Carm1 Antibody (NB100-56374) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Carm1 Antibody (NB100-56374)

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Blogs on Carm1.

Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi...  Read full blog post.

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Bioinformatics

Gene Symbol CARM1
Entrez
Uniprot